Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from Raji cells, using Phospho-B-Raf-S445 Rabbit pAb (A0038). Raji cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-S445 antibody (STJ22042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-B-Raf-S728 Rabbit pAb (STJ29342). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BRAF-S728 antibody (STJ29342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-BRAF-Ser-446 antibody (STJA0005559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BRAF-Ser446 antibody (411-460 aa) (STJ90940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-Raf-B (S602) Polyclonal Antibody

Anti-Phospho-BRAF-Ser602 antibody (576-625 aa) (STJ90876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (STJ11102615) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-T401 antibody [S5MR] (STJ11102615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Phospho-BRAF Thr401 antibody western blot K562 C6 3T3 HeLa STJA0033920

Anti-Phospho-BRAF-Thr401 antibody [R05-9B6] (STJA0033920)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using B-RAF (Phospho-Thr599) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRAF-Thr599 antibody (571-620 aa) (STJ91139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells treated with EGF 200 ng/ml for 30', using B-Raf (Phospho-Thr753) STJ90621

Anti-Phospho-BRAF-Thr753 antibody (717-766 aa) (STJ90621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Lane B &C) Western blot with Anti-Phospho-BTK Antibody (STJ502176) on MDAMB281 cells (A), and TPH cells (C) at 1:500 dilution in buffer.

Anti-Phospho-BTK antibody (544-561) (STJ502176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£497.50

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Anti-Phospho-BTK antibody (544-561) {Biotin} (STJ502177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£551.50

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Anti-Phospho-BTK antibody (544-561) {FITC} (STJ502178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£551.50

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Western Blot analysis of mouse heart, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BTK-Ser179 antibody (STJA0010049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-BTK-Ser180 antibody (STJ196255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Btk (Y223) Polyclonal Antibody

Anti-Phospho-BTK-Tyr223 antibody (188-237 aa) (STJ90447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-BTK antibody western blot Raji lysates STJA0035963

Anti-Phospho-BTK-Tyr223 antibody [R09-4V4] (STJA0035963)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various cells using Phospho-Btk (Y551) Polyclonal Antibody

Anti-Phospho-BTK-Tyr551 antibody (516-565 aa) (STJ90448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-BTK Tyr551 antibody western blot Ramos lysates STJA0036067

Anti-Phospho-BTK-Tyr551 antibody [R07-9C1] (STJA0036067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (STJ11102633) at 1:500 dilution. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-BTK-Y223 antibody [S3MR] (STJ11102633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Phospho-c-Myb Ser11 antibody western blot Jurkat STJA0034999

Anti-Phospho-c Myb-Ser11 antibody [R04-2D3] (STJA0034999)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ChIP

Reactivity :

Human

£133.50 - £281.50

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Western blot K-562 cells c-Abl phosphorylation detection using antibody STJA0041063

Anti-Phospho-c-Abl-Tyr-245 antibody (STJA0041063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot K-562 cells c-Abl phosphorylation detection using antibody STJA0041064

Anti-Phospho-c-Abl-Tyr-412 antibody (STJA0041064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis human Jurkat cells using anti-c-Cbl Tyr-700 antibody STJA0041109

Anti-Phospho-c-Cbl-Tyr-700 antibody [M161] (STJA0041109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot C-Raf detection in Jurkat cells with phosphatase treatment comparison STJA0041368

Anti-Phospho-C-Raf-Ser-471 [S578/S433] antibody (STJA0041368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot c-Src phosphorylation in mouse SYF cells with pervanadate treatment STJA0041394

Anti-Phospho-c-Src-Tyr-215 antibody (STJA0041394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis c-Src phosphorylation in SYF cells using antibody STJA0041379

Anti-Phospho-c-Src-Tyr-530 antibody (C-Term) [M261] (STJA0041379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using CD88/C5aR (Phospho-Ser338) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-C5AR1-Ser338 antibody (301-350 aa) (STJ91142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse brain using Phospho-Camk2a-T286 Rabbit mAb (STJ11107739) at 1:5000 dilution incubated overnight at 4C. Mouse brain were treated by CIP (20uL/400ul) at 37C for 1 hour. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Enhanced Kit <br>Exposure time: 180s.

Anti-Phospho-Camk2a-T286 antibody (300-400 aa) [SRM5548] (STJ11107739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£143.50 - £618.50

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Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (STJ22047) at 1:4000 dilution. Mouse brain cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Camk2a-T286 antibody (STJ22047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CAMK4-Thr200 antibody (166-215 aa) (STJ90203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of Jurkat cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CAMKK1-Ser458/495 antibody (STJA0009937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Bifidobacterium animalis subsp. lactis A6 Enhances Fatty Acid β-Oxidation of Adipose Tissue to Ameliorate the Development of Obesity in Mice Nutrients. 2022 Jan;14 (3) :598. WB mouse epididymal adipose tissues

Anti-Phospho-CAMKK2-Ser511 antibody (STJ196261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-Cannabinoid Receptor I Ser316 antibody western blot HeLa STJA0034105

Anti-Phospho-Cannabinoid Receptor I-Ser316 antibody [R05-9A1] (STJA0034105)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-P Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CARM1-S228 antibody (STJ22048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-CASC5-T943 antibody [pMELT-KNL1] (STJA0019176)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CASP2-Ser157 antibody (123-172 aa) (STJ91157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of HBE using p-Caspase-3 (S150) antibody. Antibody was diluted at 1:1000

Anti-Phospho-CASP3-Ser150 antibody (116-165 aa) (STJ90887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Caspase 8 (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CASP8-Ser347 antibody (313-362 aa) (STJ90205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Caspase-8 (Y380) Polyclonal Antibody

Anti-Phospho-CASP8-Tyr380 antibody (346-395 aa) (STJ90563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of 1 Raji cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CASP8-Tyr448 antibody (STJA0010036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, using Caspase 9 (Phospho-Ser144) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CASP9-Ser144 antibody (110-159 aa) (STJ90886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Caspase-9 (S196) Polyclonal Antibody diluted at 1:1000 STJ90505

Anti-Phospho-CASP9-Ser196 antibody (162-211 aa) (STJ90505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CASP9-Thr125 antibody (91-140 aa) (STJ90758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from COLO205 cells, using Caspase 9 (Phospho-Tyr153) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CASP9-Tyr153 antibody (119-168 aa) (STJ90702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from LOVO cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CASR-Thr888 antibody (854-903 aa) (STJ90701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-CAV1-Tyr-14 antibody [M283] (STJA0005314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of various cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CAV1-Tyr14 antibody (5-54 aa) (STJ90210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using Phospho-Caveolin-1-Y14 Rabbit pAb (STJ116375) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-CAV1-Y14 antibody (STJ116375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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