• Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using CaMK4 (Phospho-Thr196/200) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CAMK4-Thr200 antibody (166-215 aa) (STJ90203)

SKU:
STJ90203

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Calcium/calmodulin-dependent protein kinase type IV-Thr200 (166-215 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAMK4
Gene ID: 814
Uniprot ID: KCC4_HUMAN
Immunogen Region: 166-215 aa
Specificity: Phospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at T200.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CaMK4 around the phosphorylation site of Thr196/200 at the amino acid range 166-215
Post Translational Modifications Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13. Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.
Function Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Protein Name Calcium/Calmodulin-Dependent Protein Kinase Type Iv
Camk Iv
Cam Kinase-Gr
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-2151201
Reactome: R-HSA-442729
Reactome: R-HSA-9022535
Reactome: R-HSA-9022692
Reactome: R-HSA-9617324
Cellular Localisation Cytoplasm
Nucleus
Localized In Hippocampal Neuron Nuclei
In Spermatids
Associated With Chromatin And Nuclear Matrix
Alternative Antibody Names Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Iv antibody
Anti-Camk Iv antibody
Anti-Cam Kinase-Gr antibody
Anti-CAMK4 antibody
Anti-CAMK antibody
Anti-CAMK-GR antibody
Anti-CAMKIV antibody

Information sourced from Uniprot.org

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