Signal Transduction | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-STAT3-Y705 Rabbit monoclonal antibody (STJ11105819) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-STAT3-Y705 antibody [S5819RM] (STJ11105819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-Phospho-STAT3-Y705 antibody [SAA0302] (STJA0019171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€473.13 - €2,040.63

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Western blot analysis of lysates from K562 cells using Phospho-STAT5A-S780 Rabbit pAb (STJ11105769). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-STAT5A-S780 antibody (STJ11105769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€161.88 - €803.13

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Western Blot analysis of hela 293T using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-STAT5A-Ser725/730 antibody (STJA0009943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of Stat5a (phospho Ser780) Polyclonal Antibody, using Hela, Jurkat cell treated or untreated with PMA 125ng/ml 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-STAT5A-Ser780 antibody (745-794 aa) (STJ90413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-STAT5A-Tyr-694 antibody [M148] (STJA0005608)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€493.13

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Western blot analysis of lysates from TF-1 cells, using Phospho-STAT5A-Y694 Rabbit pAb (STJ22417) at 1:1000 dilution. TF-1 cells were treated by G-CSF (25 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT5A-Y694 antibody (STJ22417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-STAT5B-Tyr694 antibody (STJ196442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-Phospho-STAT6 antibody (610-660) (STJ502702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€660.63

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Anti-Phospho-STAT6 antibody (610-660) {Biotin} (STJ502703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€730.63

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Anti-Phospho-STAT6 antibody (610-660) {FITC} (STJ502704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

€730.63

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Western blot analysis of various cells using Phospho-Stat6 (T645) Polyclonal Antibody

Anti-Phospho-STAT6-Thr645 antibody (612-661 aa) (STJ90415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of Stat6 (phospho Tyr641) Polyclonal Antibody, using Hela, Jurkat cell treated or untreated with IFN 2500U/ml 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-STAT6-Tyr641 antibody (608-657 aa) (STJ90416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-STAT6-Tyr641 antibody [PT0917R] (STJA0039807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa cells, using Phospho-STK11-S334 Rabbit pAb (STJ29367) at 1:1000 dilution. Hela cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-STK11-S334 antibody (STJ29367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK11-S428 Rabbit pAb (STJ11101142) at 1:1000 dilution or LKB1 antibody (STJ25732). NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STK11-S428 antibody (STJ11101142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of HeLa cells, using Phospho-STK11-S428 antibody (STJ29368). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-STK11-S428 antibody (STJ29368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STK11-Ser334 antibody (300-349 aa) (STJ91208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, LKB1 (phospho Ser428) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STK11-Ser428 antibody (384-433 aa) (STJ91060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Cow

€60.00 - €315.00

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Western blot analysis of lysates from A-431 cells, using Phospho-STK11-T189 Rabbit pAb (STJ25732). A-431 cells were treated by 10% FBS for after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit

Anti-Phospho-STK11-T189 antibody (STJ29369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of 3T3 cells using Phospho-LKB1 (T189) Polyclonal Antibody

Anti-Phospho-STK11-Thr189 antibody (155-204 aa) (STJ90488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK3/MST2-T117 Rabbit pAb (STJ11101068) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-STK3-T117 antibody (STJ11101068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various lysates using Phospho-STK3/MST2-T384 Rabbit pAb (STJ11101069) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-STK3-T384 antibody (STJ11101069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western Blot analysis of 1 MCF-7 cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-STK38-Thr444/442 antibody (STJA0009963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from NIH/3T3 and C6 cells, using phospho-MST1/STK4-T387 pAb (STJ11101041) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-STK4-T387 antibody (STJ11101041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from HeLa and C2C12 cells, using Phospho-Stathmin 1-S16 Rabbit pAb (STJ22423) at 1:2000 dilution. HeLa and C2C12 cells were treated by Nocodazole (50ng/mL) for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-STMN1-S16 antibody (STJ22423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 Rabbit pAb (STJ22421) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STMN1-S25 antibody (STJ22421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of C6, using Phospho-Stathmin 1-S25 Rabbit monoclonal antibody (STJ11105891) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-STMN1-S25 antibody [S5891RM] (STJ11105891)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€183.13 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Stathmin 1-S38 Rabbit pAb (STJ22422) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-STMN1-S38 antibody (STJ22422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using Phospho-Op18 (S16) Polyclonal Antibody

Anti-Phospho-STMN1-Ser16 antibody (5-54 aa) (STJ90357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-Op18 (S25) Polyclonal Antibody

Anti-Phospho-STMN1-Ser25 antibody (9-58 aa) (STJ90358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-Op18 (S38) Polyclonal Antibody

Anti-Phospho-STMN1-Ser38 antibody (5-54 aa) (STJ90359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using STMN1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-STMN1-Ser62 antibody (26-75 aa) (STJ90936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-STMN2-Ser16 antibody (STJ194748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-STMN2-Ser38 antibody (STJ194749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-STMN2-Ser73 antibody (STJ91270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-Survivin antibody (STJ502705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-Phospho-Survivin antibody {Biotin} (STJ502706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€730.63

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Anti-Phospho-Survivin antibody {FITC} (STJ502707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€730.63

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SYK-Ser297 antibody (STJ196443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HepG2 cells, using SYK (Phospho-Tyr323) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SYK-Tyr323 antibody (289-338 aa) (STJ91056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-Syk (Y348) Polyclonal Antibody

Anti-Phospho-SYK-Tyr348 antibody (314-363 aa) (STJ90774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-SYK-Tyr348 antibody (STJ99593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-SYK-Tyr352 antibody (STJ90660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-Syk (Y525) Polyclonal Antibody

Anti-Phospho-SYK-Tyr525 antibody (491-540 aa) (STJ90775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Anti-Phospho-Syk-Tyr525/526 antibody [PT0753R] (STJA0039170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from U-937 cells using Phospho-Syk-Y323 Rabbit pAb (STJ11103715) at1:500 dilution. U-937 cells were treated by ATP (5 mM) at 30C for 1 hour. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.

Anti-Phospho-Syk-Y323 antibody (STJ11103715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (STJ22425). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYK-Y323 antibody (STJ22425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

€161.88 - €803.13

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