• Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody

Anti-Phospho-ARHGAP35-Tyr1105 antibody (1071-1120 aa) (STJ90795)

SKU:
STJ90795

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Rho GTPase-activating protein 35-Tyr1105 (1071-1120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP35
Gene ID: 2909
Uniprot ID: RHG35_HUMAN
Immunogen Region: 1071-1120 aa
Specificity: Phospho-GRF-1 (Y1105) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1105.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GRF-1 around the phosphorylation site of Tyr1105 at the amino acid range 1071-1120
Post Translational Modifications Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. In brain, phosphorylated by FYN and SRC. During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin. Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration.
Function Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis. Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation. Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation. During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth. Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'.this function is however unclear and would need additional experimental evidences.
Protein Name Rho Gtpase-Activating Protein 35
Glucocorticoid Receptor Dna-Binding Factor 1
Glucocorticoid Receptor Repression Factor 1
Grf-1
Rho Gap P190a
P190-A
Database Links Reactome: R-HSA-416550
Reactome: R-HSA-8849471
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Nucleus
Cell Membrane
In Response To Integrins And Sdc4 And Upon Phosphorylation By Pkc
Relocalizes From The Cytoplasm To Regions Of Plasma Membrane Ruffling Where It Colocalizes With Polymerized Actin
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 35 antibody
Anti-Glucocorticoid Receptor Dna-Binding Factor 1 antibody
Anti-Glucocorticoid Receptor Repression Factor 1 antibody
Anti-Grf-1 antibody
Anti-Rho Gap P190a antibody
Anti-P190-A antibody
Anti-ARHGAP35 antibody
Anti-GRF1 antibody
Anti-GRLF1 antibody
Anti-KIAA1722 antibody
Anti-P190A antibody
Anti-p190ARHOGAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance