• Western blot analysis of lysates from HT29 cells treated with Insulin 0.01U/ML 15', using ATF1 (Phospho-Ser63) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of Mouse-kidney KB 293T 3T3 lysis using Phospho-ATF-1 (S63) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ATF1-Ser63 antibody (31-80 aa) (STJ90454)

SKU:
STJ90454

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cyclic AMP-dependent transcription factor ATF-1-Ser63 (31-80 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF1
Gene ID: 466
Uniprot ID: ATF1_HUMAN
Immunogen Region: 31-80 aa
Specificity: Phospho-ATF-1 (S63) Polyclonal Antibody detects endogenous levels of ATF-1 protein only when phosphorylated at S63.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ATF1 around the phosphorylation site of Ser63 at the amino acid range 31-80
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-1
Camp-Dependent Transcription Factor Atf-1
Activating Transcription Factor 1
Protein Treb36
Database Links Reactome: R-HSA-199920
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-1 antibody
Anti-Camp-Dependent Transcription Factor Atf-1 antibody
Anti-Activating Transcription Factor 1 antibody
Anti-Protein Treb36 antibody
Anti-ATF1 antibody

Information sourced from Uniprot.org

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