• Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30', using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARHGEF2-Ser885 antibody (851-900 aa) (STJ90558)

SKU:
STJ90558

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Rho guanine nucleotide exchange factor 2-Ser885 (851-900 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF2
Gene ID: 9181
Uniprot ID: ARHG2_HUMAN
Immunogen Region: 851-900 aa
Specificity: Phospho-Lfc (S885) Polyclonal Antibody detects endogenous levels of Lfc protein only when phosphorylated at S885.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of Ser885 at the amino acid range 851-900
Function Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans.acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability. Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons.
Protein Name Rho Guanine Nucleotide Exchange Factor 2
Guanine Nucleotide Exchange Factor H1
Gef-H1
Microtubule-Regulated Rho-Gef
Proliferating Cell Nucleolar Antigen P40
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Tight Junction
Golgi Apparatus
Spindle
Cell Projection
Ruffle Membrane
Cytoplasmic Vesicle
Localizes To The Tips Of Cortical Microtubules Of The Mitotic Spindle During Cell Division
And Is Further Released Upon Microtubule Depolymerization
Recruited Into Membrane Ruffles Induced By S
Flexneri At Tight Junctions Of Polarized Epithelial Cells
Colocalized With Nod2 And Ripk2 In Vesicles And With The Cytoskeleton
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 2 antibody
Anti-Guanine Nucleotide Exchange Factor H1 antibody
Anti-Gef-H1 antibody
Anti-Microtubule-Regulated Rho-Gef antibody
Anti-Proliferating Cell Nucleolar Antigen P40 antibody
Anti-ARHGEF2 antibody
Anti-KIAA0651 antibody
Anti-LFP40 antibody

Information sourced from Uniprot.org

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