Protein Localisation | Research Area | St John's Laboratory

Western blot analysis of extracts from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa cells, using TTC11/FIS1 antibody (STJ11104392) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FIS1 antibody (1-122) (STJ11104392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using TTC11/FIS1 Rabbit pAb (STJ28384) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-FIS1 antibody (1-122) (STJ28384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g TTC11/FIS1 antibody (STJ11101755). Western blot was performed from the immunoprecipitate using TTC11/FIS1 (STJ11101755) at a dilution of 1:2000.

Anti-FIS1 antibody (1-122) [S5MR] (STJ11101755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-FIS1 antibody (1-122aa) [AT3B7] (STJA0041863)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-FIS1 antibody (27-77) (STJA0006648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunocytochemistry analysis of TTC11/FIS1 (green) in MCF-7 using TTC11/FIS1 antibody, and DAPI (blue)

Anti-FIS1 antibody (8-21 aa) [R02-8G8] (STJA0012090)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Frataxin antibody (42-210aa) [AT10E11] (STJA0041814)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-Frataxin/FXN antibody [SRM14827] (STJ11109557)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of Hep G2 cells using FXN/ Frataxin Rabbit polyclonal antibody (STJ113368) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FXN antibody (42-210) (STJ113368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of FXN/ Frataxin in paraffin-embedded human liver cancer using FXN/ Frataxin Rabbit polyclonal antibody (STJ23722) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-FXN antibody (42-210) (STJ23722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-FXN antibody (STJA0006974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using GDAP1 antibody (STJ28684) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GDAP1 antibody (1-225) (STJ28684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GDAP1 antibody (STJA0009116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-GET3 antibody (112-162) (STJA0006636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using GET4 Rabbit polyclonal antibody (STJ118630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GET4 antibody (1-327) (STJ118630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GET4 antibody (190-240 aa) (STJ193920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of HBE cells using ALR Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GFER antibody (51-100 aa) (STJ96894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of GFER in paraffin-embedded Mouse brain tissue using GFER Rabbit polyclonal antibody (STJ27416) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-GFER antibody (81-205) (STJ27416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-GNPAT antibody (231-280 aa) (STJ93304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Grp75 antibody [4I9] (STJ16103543)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-GRP75 antibody [R06-1W5] (STJA0036518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of mouse liver, using GRPEL1 rabbit polyclonal antibody (STJ27014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GRPEL1 antibody (1-217) (STJ27014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GRPEL1 antibody (159-209 aa) (STJ194944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using GRPEL2 Rabbit pAb (STJ110637) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GRPEL2 antibody (33-225) (STJ110637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GRPEL2 antibody (42-92 aa) (STJ196140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using GSTK1 Rabbit mAb (STJ11102523) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSTK1 antibody (1-100) [S3MR] (STJ11102523)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells, using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11100839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GSTK1 antibody (1-226) (STJ11100839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSTK1 antibody (1-226) (STJ11104362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using GSTK1 Rabbit polyclonal antibody (STJ23885) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSTK1 antibody (1-226) (STJ23885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GSTK1 antibody (138-188 aa) (STJ195875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-GSTK1 antibody [R03-9F4] (STJA0034374)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£140.50 - £308.50

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Anti-GSTK1 antibody [R04-3X6] (STJA0037051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HACL1 antibody (323-373 aa) (STJ196113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of paraffin-embedded rat liver using HAO1/GOX Rabbit polyclonal antibody (STJ28553) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HAO1 antibody (1-370) (STJ28553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HAO1 antibody (STJ197373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Chk2 (phospho Thr68) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HAO1 antibody [Mix] (STJ97395)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£49.00 - £261.00

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Anti-HAO1/GOX antibody [SRM14475] (STJ11109205)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Heme Oxygenase 1 antibody [R02-6C1] (STJA0034405)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Heme Oxygenase 1 antibody [R03-8A2] (STJA0033875)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse

£133.50 - £281.50

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Anti-Heme Oxygenase 1 antibody [R09-2O1] (STJA0035785)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of various lysates using HMGCL Rabbit pAb (STJ110355) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 15s.

Anti-HMGCL antibody (1-325) (STJ110355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HMGCL antibody (C-Term) (STJA0010324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGCL antibody [R08-7H5] (STJA0034422)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded Rat spleen using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ113706) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody (1-265) (STJ113706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of Raw264. 7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11101600) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HMOX1 antibody (1-265) [S0MR] (STJ11101600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates, using HemeOxygenase1 (HO-1/HMOX1) antibody (STJ11104601) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX1 antibody (1-265) [S4601RM] (STJ11104601)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ116419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ116419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 Cell lysate using HO-1 mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HMOX1 antibody [Mix] (STJ196663)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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