Anti-HAO1 antibody (1-370) (STJ28553)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HAO1 (1-370) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HAO1 |
| Gene ID | 54363 |
| Uniprot ID | HAOX1_HUMAN |
| Immunogen Region | 1-370 |
| Immunogen Sequence | MLPRLICINDYEQHAKSVLP KSIYDYYRSGANDEETLADN IAAFSRWKLYPRMLRNVAET DLSTSVLGQRVSMPICVGAT AMQRMAHVDGELATVRACQS LGTGMMLSSWATSSIEEVAE AGPEALRWLQLYIYKDREVT KKLVRQAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQ LRMKNFETSTLSFSPEENFG DDSGLAAYVAKAIDPSISWE DIKWLRRLTSLPIVAKGIL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1/GOX (NP_060015.1). |
Additional Info
| Tissue Specificity | Highly expressed in liver. |
| Function | Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine.this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency. Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on L-lactate and 2-hydroxybutanoate. |
| Protein Name | 2-Hydroxyacid Oxidase 1Haox1Glycolate OxidaseGoGoxGlyoxylate Oxidase |
| Database Links | Reactome: R-HSA-389661Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome Matrix |
| Alternative Antibody Names | Anti-2-Hydroxyacid Oxidase 1 antibodyAnti-Haox1 antibodyAnti-Glycolate Oxidase antibodyAnti-Go antibodyAnti-Gox antibodyAnti-Glyoxylate Oxidase antibodyAnti-HAO1 antibodyAnti-GOX1 antibody |
Information sourced from Uniprot.org