• Western blot analysis of extracts of various cell lines, using GRPEL2 antibody (STJ110637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using GRPEL2 Rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using GRPEL2 rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GRPEL2 Rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using GRPEL2 rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using GRPEL2 Rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using GRPEL2 rabbit polyclonal antibody (STJ110637) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using GRPEL2 antibody (STJ110637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GRPEL2 antibody (33-225) (STJ110637)

SKU:
STJ110637

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRPEL2 (33-225) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRPEL2
Gene ID: 134266
Uniprot ID: GRPE2_HUMAN
Immunogen Region: 33-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-225 of human GRPEL2 (NP_689620.2).
Immunogen Sequence: STATQRTAGEDCRSEDPPDE LGPPLAERALRVKAVKLEKE VQDLTVRYQRAIADCENIRR RTQRCVEDAKIFGIQSFCKD LVEVADILEKTTECISEESE PEDQKLTLEKVFRGLLLLEA KLKSVFAKHGLEKLTPIGDK YDPHEHELICHVPAGVGVQP GTVALVRQDGYKLHGRTIRL ARVEVAVESQRRL
Function Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70.
Protein Name Grpe Protein Homolog 2 - Mitochondrial
Mt-Grpe#2
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Grpe Protein Homolog 2 - Mitochondrial antibody
Anti-Mt-Grpe#2 antibody
Anti-GRPEL2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance