![Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177006/368175/STJ11104362_1__53400.1713133685.jpg?c=1 "Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177006/368176/STJ11104362_2__67511.1713133685.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.")
![Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177006/368177/STJ11104362_3__28808.1713133686.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.")
![Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177006/368175/STJ11104362_1__53400.1713133685.jpg?c=1 "Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-GSTK1 antibody (1-226) (STJ11104362)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Glutathione S-Transferase Kappa 1 (1-226) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSTK1 |
| Gene ID | 373156 |
| Uniprot ID | GSTK1_HUMAN |
| Immunogen Region | 1-226 |
| Immunogen Sequence | MGPLPRTVELFYDVLSPYSW LGFEILCRYQNIWNINLQLR PSLITGIMKDSGNKPPGLLP RKGLYMANDLKLLRHHLQIP IHFPKDFLSVMLEKGSLSAM RFLTAVNLEHPEMLEKASRE LWMRVWSRNEDITEPQSILA AAEKAGMSAEQAQGLLEKIA TPKVKNQLKETTEAACRYGA FGLPITVAHVDGQTHMLFGS DRMELLAHLLGEKWMGPIPP AVNARL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Glutathione S-transferase that catalyzes the conjugation of glutathione to exogenous and endogenous compounds. Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). |
| Protein Name | Glutathione S-Transferase Kappa 1Gst 13-13Gst Class-KappaGstk1-1Hgstk1Glutathione S-Transferase Subunit 13 |
| Database Links | Reactome: R-HSA-156590Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome |
| Alternative Antibody Names | Anti-Glutathione S-Transferase Kappa 1 antibodyAnti-Gst 13-13 antibodyAnti-Gst Class-Kappa antibodyAnti-Gstk1-1 antibodyAnti-Hgstk1 antibodyAnti-Glutathione S-Transferase Subunit 13 antibodyAnti-GSTK1 antibodyAnti-HDCMD47P antibody |
Information sourced from Uniprot.org