• Immunohistochemistry analysis of GFER in paraffin-embedded Mouse brain tissue using GFER Rabbit polyclonal antibody (STJ27416) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GFER in paraffin-embedded Mouse spleen tissue using GFER Rabbit polyclonal antibody (STJ27416) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GFER in paraffin-embedded Rat brain tissue using GFER Rabbit polyclonal antibody (STJ27416) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using GFER Rabbit polyclonal antibody (STJ27416) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GFER antibody (81-205) (STJ27416)

SKU:
STJ27416

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GFER (81-205) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFER
Gene ID: 2671
Uniprot ID: ALR_HUMAN
Immunogen Region: 81-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER (NP_005253.3).
Immunogen Sequence: MRTQQKRDTKFREDCPPDRE ELGRHSWAVLHTLAAYYPDL PTPEQQQDMAQFIHLFSKFY PCEECAEDLRKRLCRNHPDT RTRACFTQWLCHLHNEVNRK LGKPDFDCSKVDERWRDGWK DGSCD
Tissue Specificity Ubiquitously expressed. Highest expression in the testis and liver and low expression in the muscle.
Function Isoform 1: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. Isoform 2: May act as an autocrine hepatotrophic growth factor promoting liver regeneration.
Protein Name Fad-Linked Sulfhydryl Oxidase Alr
Augmenter Of Liver Regeneration
Herv1
Hepatopoietin
Database Links Reactome: R-HSA-1268020
Cellular Localisation Isoform 1: Mitochondrion Intermembrane Space
Mitochondrion
Isoform 2: Cytoplasm
Secreted
Alternative Antibody Names Anti-Fad-Linked Sulfhydryl Oxidase Alr antibody
Anti-Augmenter Of Liver Regeneration antibody
Anti-Herv1 antibody
Anti-Hepatopoietin antibody
Anti-GFER antibody
Anti-ALR antibody
Anti-HERV1 antibody
Anti-HPO antibody

Information sourced from Uniprot.org

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