• Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using GSTK1 Rabbit polyclonal antibody (STJ23885) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using GSTK1 Rabbit polyclonal antibody (STJ23885) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using GSTK1 Rabbit polyclonal antibody (STJ23885) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GSTK1 antibody (STJ23885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GSTK1 antibody (1-226) (STJ23885)

SKU:
STJ23885

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GSTK1 (1-226) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTK1
Gene ID: 373156
Uniprot ID: GSTK1_HUMAN
Immunogen Region: 1-226
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).
Immunogen Sequence: MGPLPRTVELFYDVLSPYSW LGFEILCRYQNIWNINLQLR PSLITGIMKDSGNKPPGLLP RKGLYMANDLKLLRHHLQIP IHFPKDFLSVMLEKGSLSAM RFLTAVNLEHPEMLEKASRE LWMRVWSRNEDITEPQSILA AAEKAGMSAEQAQGLLEKIA TPKVKNQLKETTEAACRYGA FGLPITVAHVDGQTHMLFGS DRMELLAHLLGEKWMGPIPP AVNARL
Tissue Specificity Ubiquitous.
Function Glutathione S-transferase that catalyzes the conjugation of glutathione to exogenous and endogenous compounds. Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
Protein Name Glutathione S-Transferase Kappa 1
Gst 13-13
Gst Class-Kappa
Gstk1-1
Hgstk1
Glutathione S-Transferase Subunit 13
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Glutathione S-Transferase Kappa 1 antibody
Anti-Gst 13-13 antibody
Anti-Gst Class-Kappa antibody
Anti-Gstk1-1 antibody
Anti-Hgstk1 antibody
Anti-Glutathione S-Transferase Subunit 13 antibody
Anti-GSTK1 antibody
Anti-HDCMD47P antibody

Information sourced from Uniprot.org

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