| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HMGCL (1-325) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HMGCL |
| Gene ID: |
3155 |
| Uniprot ID: |
HMGCL_HUMAN |
| Immunogen Region: |
1-325 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human HMGCL (NP_000182.2). |
| Immunogen Sequence: |
MAAMRKALPRRLVGLASLRA VSTSSMGTLPKRVKIVEVGP RDGLQNEKNIVSTPVKIKLI DMLSEAGLSVIETTSFVSPK WVPQMGDHTEVLKGIQKFPG INYPVLTPNLKGFEAAVAAG AKEVVIFGAASELFTKKNIN CSIEESFQRFDAILKAAQSA NISVRGYVSCALGCPYEGKI SPAKVAEVTKKFYSMGCYEI SLGDTIGVGTPGIMKDMLSA VMQEVPLAALAVHCHDTYG |
| Tissue Specificity | Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%). |
| Function | Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. |
| Protein Name | Hydroxymethylglutaryl-Coa Lyase - MitochondrialHlHmg-Coa Lyase3-Hydroxy-3-Methylglutarate-Coa Lyase |
| Database Links | Reactome: R-HSA-77111Reactome: R-HSA-9033241 |
| Cellular Localisation | Mitochondrion MatrixPeroxisomeUnprocessed Form Is Peroxisomal |
| Alternative Antibody Names | Anti-Hydroxymethylglutaryl-Coa Lyase - Mitochondrial antibodyAnti-Hl antibodyAnti-Hmg-Coa Lyase antibodyAnti-3-Hydroxy-3-Methylglutarate-Coa Lyase antibodyAnti-HMGCL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98772/382820/STJ119987_1__54161.1713151317.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")