Neuronal System | Research Area | St John's Laboratory

Western blot analysis of 3T3 cells using Phospho-PKC Alpha (T638) Polyclonal Antibody

Anti-Phospho-PRKCA-Thr638 antibody (606-655 aa) (STJ90863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKCA-Thr638/641 antibody (STJ196382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-PKC Alpha (Y657) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCA-Tyr657 antibody (623-672 aa) (STJ90499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HeLa cells treated with heat shock, using PKCB (Phospho-Ser661) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCB-Ser661 antibody (622-671 aa) (STJ90592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-PRKCB-T641 antibody (STJ11103725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (STJ22364) at 1:1000 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RAC1-S71 antibody (STJ22364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western blot analysis of various lysates using (STJ116387) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S363 antibody (STJ116387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (STJ113499) at 1:1000 dilution or RPS6KA1 antibody (STJ240768). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S380 antibody (STJ113499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (STJ11102641) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-RPS6KA1-S380 antibody [S1MR] (STJ11102641)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-RPS6KA1-Ser227 antibody (STJ196410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-Rsk-1 (S380) Polyclonal Antibody

Anti-Phospho-RPS6KA1-Ser380 antibody (346-395 aa) (STJ90474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of HeLa, using Phospho-p90Rsk/RSK1/RPS6KA1-T348 antibody (STJ11105866) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-RPS6KA1-T348 antibody (STJ11105866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from A431 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 Rabbit pAb (STJ113490). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RPS6KA1-T359/S363 antibody (STJ113490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-RPS6KA1-T573 antibody (STJ11101137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Thr359+Ser363) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KA1-Thr359/S363 antibody (331-380 aa) (STJ90825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-RPS6KA1-Thr359/Ser363 antibody (STJ196373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-RPS6KA1-Thr573 antibody (539-588 aa) (STJ91047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-RPS6KA2-Thr353 antibody (STJA0009881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RPS6KA3-Tyr529 antibody (STJA0009989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-Src antibody (200-300) (STJ502687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-Phospho-Src antibody (200-300) {Biotin} (STJ502689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (200-300) {FITC} (STJ502690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (400-500) (STJ502688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-Phospho-Src antibody (400-500) {Biotin} (STJ502691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (400-500) {FITC} (STJ502692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Western blot analysis of lysates from 293T cells, using Phospho-Src-S17 Rabbit pAb (STJ113487) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SRC-S17 antibody (STJ113487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-SRC-Ser17 antibody (STJ196435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of COLO cells using Phospho-c-Src (S75) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Ser75 antibody (41-90 aa) (STJ90958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SRC-Tyr-215 antibody (STJA0005602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$496.13

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Anti-Phospho-SRC-Tyr-530 antibody (C-Term) [M261] (STJA0005603)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$496.13

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using Src (Phospho-Tyr216) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SRC-Tyr216 antibody (181-230 aa) (STJ90611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of Jurkat cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SRC-Tyr419 antibody (386-435 aa) (STJ90237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Tyr419 antibody (STJ90446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-SRC-Tyr529 antibody (487-536 aa) (STJ90238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (STJ11102608) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SRC-Y419 antibody [S8MR] (STJ11102608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (STJ22407) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SRC-Y529 antibody (STJ22407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of A-431, using Phospho-Src-Y529 antibody (STJ11105854) at 1:10000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SRC-Y529 antibody [S5854RM] (STJ11105854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STX1A-Ser14 antibody (1-50 aa) (STJ90729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-STXBP1-Ser313 antibody (279-328 aa) (STJ90471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from mouse Brain tissue using Phospho-SYN1-S9 Rabbit pAb (STJ22426). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYN1-S9 antibody (STJ22426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of 293T using SYN1 p-S553 antibody. Antibody was diluted at 1:500

Anti-Phospho-SYN1-Ser553 antibody (STJ97692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Synapsin1 (Phospho-Ser605) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser605 antibody (576-625 aa) (STJ91309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HeLa cells treated with Anisomycin 25ug/ml 30', using Synapsin1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser62 antibody (26-75 aa) (STJ90832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Synapsin I (S9) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SYN1-Ser9 antibody (3-52 aa) (STJ90417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$496.13

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using PICK1 antibody (STJ115535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PICK1 antibody (300-400) (STJ115535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using PICK1 antibody (STJ24986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PICK1 antibody (300-400) (STJ24986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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