Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using PSMD9 antibody (STJ27310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.

Anti-PSMD9 antibody (1-223) (STJ27310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PSME1 Rabbit monoclonal antibody (STJ11102239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME1 antibody (1-115) [S9MR] (STJ11102239)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using PSME1 antibody (STJ27311) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-PSME1 antibody (1-249) (STJ27311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and PSME2 knockout (KO) 293T cells, using [KO Validated] PSME2 Rabbit polyclonal antibody (STJ11100740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ11100740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts from wild type (WT) and PSME2 knockout (KO) 293T (KO) cells, using PSME2 antibody (STJ115590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ115590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts from wild type (WT) and PSME2 knockout (KO) 293T (KO) cells, using PSME2 antibody (STJ27508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ27508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunohistochemistry analysis of PSME3 in paraffin-embedded rat kidney using PSME3 Rabbit polyclonal antibody (STJ114570) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PSME3 antibody (200-254) (STJ114570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PSME3 antibody (200-254) (STJ119994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of PSME4 in paraffin-embedded Human esophageal using PSME4 Rabbit polyclonal antibody (STJ115757) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PSME4 antibody (1634-1843) (STJ115757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PSMF1 rabbit polyclonal antibody (STJ27500) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMF1 antibody (1-271) (STJ27500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PSPH antibody (STJ11104949) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSPH antibody (1-225 aa) [S4949RM] (STJ11104949)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using PSPH Rabbit pAb (STJ110233) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PSPH antibody (1-225) (STJ110233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PSPH antibody (STJA0006792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PTDSS2 antibody [PT0795R] (STJA0039356)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/IP

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using PTEN antibody (STJ113730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTEN antibody (1-200) (STJ113730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using PTEN Rabbit polyclonal antibody (STJ29771) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTEN antibody (1-403) (STJ29771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PTEN antibody (STJ29874) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PTEN antibody (190-304) (STJ29874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PTEN Mouse monoclonal antibody (STJ112974) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTEN antibody (204-403) [S4MM] (STJ112974)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human stomach using PTEN antibody (STJ113011) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PTEN antibody (224-403) (STJ113011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of human-heart tissue. 1, PTEN Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PTEN antibody (251-300 aa) (STJ98583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PTEN (PT2124) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HEK293 Lane 3: MCF7Predicted band size: 47kDaObserved band size: 47kDa

Anti-PTEN antibody (300-403) [PT2124] (STJA0006236)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PTEN Rabbit monoclonal antibody antibody (STJ11104594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTEN antibody (304-403) [S4594RM] (STJ11104594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PTEN antibody [PT0226R] (STJA0039660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-PTGDS antibody (115-165) (STJA0006385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Mouse heart, using PTGDS antibody (STJ27680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTGDS antibody (23-190) (STJ27680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of PTGES in paraffin-embedded Mouse testis using PTGES Rabbit polyclonal antibody (STJ11100566) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PTGES antibody (1-100) (STJ11100566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PGE synthase Polyclonal Antibody diluted at 1：500

Anti-PTGES antibody (30-79 aa) (STJ95054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Prostaglandin E Synthase in MCF-7, C6, 3T3, Hela lysates using Prostaglandin E Synthase antibody.

Anti-PTGES antibody (42-59 aa) [R03-8E7] (STJA0011835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of A549 cells using PTGES2 antibody (STJ115401). Blue: DAPI for nuclear staining.

Anti-PTGES2 antibody (88-377) (STJ115401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-549 cells using PTGES2 antibody (STJ29217). Blue: DAPI for nuclear staining.

Anti-PTGES2 antibody (88-377) (STJ29217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PTGES2 antibody (STJA0006972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using P23/PTGES3 Rabbit polyclonal antibody (STJ27278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PTGES3 antibody (1-100) (STJ27278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PTGES3 antibody (1-100) [S9MR] (STJ11102289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using P23/PTGES3 Rabbit polyclonal antibody (STJ115361) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PTGES3 antibody (1-110) (STJ115361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using p23 Polyclonal Antibody diluted at 1：2000

Anti-PTGES3 antibody (79-128 aa) (STJ94863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PTGIS antibody (151-500 aa) (STJ11106677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PTGIS antibody (C-Term) (STJ95055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using LTB4DH Polyclonal Antibody

Anti-PTGR1 antibody (75-124 aa) (STJ93962)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of K562 cells using PTGR2 Polyclonal Antibody

Anti-PTGR2 antibody (181-230 aa) (STJ95261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using COX1/COX1/PTGS1 Polyclonal Antibody (STJ29480) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTGS1 antibody (1-180) (STJ29480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Hela, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-PTGS1 antibody (483-533 aa) (STJ197405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates using COX1/PTGS1 Rabbit monoclonal antibody (STJ11102037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PTGS1 antibody (50-150) [S7MR] (STJ11102037)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using Cox-1 Polyclonal Antibody

Anti-PTGS1 antibody (550-599 aa) (STJ92432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded human colon using COX2/PTGS2 Rabbit polyclonal antibody (STJ111060) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PTGS2 antibody (375-604) (STJ111060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Human colon carcinoma tissue was stained with Anti-COX-2 (ABT-COX2) Antibody

Anti-PTGS2 antibody (500-604 aa) [ABT-COX2] (STJ197010)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-COX-2 (ABT040) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 70kDa

Anti-PTGS2 antibody (500-604 aa) [ABT040] (STJ197136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates, using PGHS-2 Rabbit polyclonal antibody (STJ11105324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PTGS2 antibody (505-604) (STJ11105324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of human-liver-cancer tissue. 1, Cox-2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PTGS2 antibody (555-604 aa) (STJ92438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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