• Immunofluorescence analysis of U2OS cells using PDK4 Rabbit polyclonal antibody (STJ115300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using PDK4 Rabbit polyclonal antibody (STJ115300) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using PDK4 Rabbit polyclonal antibody (STJ115300) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using PDK4 Rabbit polyclonal antibody (STJ115300) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using PDK4 antibody (STJ115300) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDK4 antibody (102-411) (STJ115300)

SKU:
STJ115300

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDK4 (102-411) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDK4
Gene ID: 5166
Uniprot ID: PDK4_HUMAN
Immunogen Region: 102-411
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 102-411 of human PDK4 (NP_002603.1).
Immunogen Sequence: FHEKSPDDQKALSDFVDTLI KVRNRHHNVVPTMAQGIIEY KDACTVDPVTNQNLQYFLDR FYMNRISTRMLMNQHILIFS DSQTGNPSHIGSIDPNCDVV AVVQDAFECSRMLCDQYYLS SPELKLTQVNGKFPDQPIHI VYVPSHLHHMLFELFKNAMR ATVEHQENQPSLTPIEVIVV LGKEDLTIKISDRGGGVPLR IIDRLFSYTYSTAPTPVMDN SRNAPLAGFGYGLPISRLY
Tissue Specificity Ubiquitous.highest levels of expression in heart and skeletal muscle.
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Protein Name Pyruvate Dehydrogenase
Acetyl-Transferring Kinase Isozyme 4 - Mitochondrial
Pyruvate Dehydrogenase Kinase Isoform 4
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase antibody
Anti-Acetyl-Transferring Kinase Isozyme 4 - Mitochondrial antibody
Anti-Pyruvate Dehydrogenase Kinase Isoform 4 antibody
Anti-PDK4 antibody
Anti-PDHK4 antibody

Information sourced from Uniprot.org

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