• Western blot analysis of extracts of SH-SY5Y cells, using PDK3 Antibody (STJ114354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PDK3 antibody (137-406) (STJ114354)

SKU:
STJ114354

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDK3 (137-406) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDK3
Gene ID: 5165
Uniprot ID: PDK3_HUMAN
Immunogen Region: 137-406
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
Immunogen Sequence: FGFDPFISTNIQYFLDRFYT NRISFRMLINQHTLLFGGDT NPVHPKHIGSIDPTCNVADV VKDAYETAKMLCEQYYLVAP ELEVEEFNAKAPDKPIQVVY VPSHLFHMLFELFKNSMRAT VELYEDRKEGYPAVKTLVTL GKEDLSIKISDLGGGVPLRK IDRLFNYMYSTAPRPSLEPT RAAPLAGFGYGLPISRLYAR YFQGDLKLYSMEGVGTDAVI YLKALSSESFERLPVFNKS
Tissue Specificity Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain.
Function Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
Protein Name Pyruvate Dehydrogenase
Acetyl-Transferring Kinase Isozyme 3 - Mitochondrial
Pyruvate Dehydrogenase Kinase Isoform 3
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase antibody
Anti-Acetyl-Transferring Kinase Isozyme 3 - Mitochondrial antibody
Anti-Pyruvate Dehydrogenase Kinase Isoform 3 antibody
Anti-PDK3 antibody
Anti-PDHK3 antibody

Information sourced from Uniprot.org

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