| Short Description : | Rabbit polyclonal PDK3 (137-406) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDK3 |
| Gene ID: | 5165 |
| Uniprot ID: | PDK3_HUMAN |
| Immunogen Region: | 137-406 |
| Immunogen Sequence: | FGFDPFISTNIQYFLDRFYT NRISFRMLINQHTLLFGGDT NPVHPKHIGSIDPTCNVADV VKDAYETAKMLCEQYYLVAP ELEVEEFNAKAPDKPIQVVY VPSHLFHMLFELFKNSMRAT VELYEDRKEGYPAVKTLVTL GKEDLSIKISDLGGGVPLRK IDRLFNYMYSTAPRPSLEPT RAAPLAGFGYGLPISRLYAR YFQGDLKLYSMEGVGTDAVI YLKALSSESFERLPVFNKS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1). |
| Tissue Specificity | Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain. |
| Function | Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species. |
| Protein Name | Pyruvate DehydrogenaseAcetyl-Transferring Kinase Isozyme 3 - MitochondrialPyruvate Dehydrogenase Kinase Isoform 3 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase antibodyAnti-Acetyl-Transferring Kinase Isozyme 3 - Mitochondrial antibodyAnti-Pyruvate Dehydrogenase Kinase Isoform 3 antibodyAnti-PDK3 antibodyAnti-PDHK3 antibody |
Information sourced from Uniprot.org
