• Flow cytometric analysis of Lovo cells using PDK1 monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) with DAB staining using PDK1 monoclonal antibody.
  • Immunofluorescence analysis of HELA cells using PDK1 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using PDK1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , Jurkat (3) , HepG2 (4) , PC-12 (5) , and Cos7 (6) cell lysate.

Anti-PDK1 antibody [4A11] (STJ98315)

SKU:
STJ98315

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Pyruvate dehydrogenase kinase isozyme 1, mitochondrial is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4A11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDK1
Gene ID: 5163
Uniprot ID: PDK1_HUMAN
Specificity: PDK1 Monoclonal Antibody detects endogenous levels of PDK1 protein.
Immunogen: Purified recombinant fragment of human PDK1 expressed in E. Coli.
Post Translational Modifications Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Protein Name Pyruvate Dehydrogenase
Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial
Pyruvate Dehydrogenase Kinase Isoform 1
Pdh Kinase 1
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase antibody
Anti-Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial antibody
Anti-Pyruvate Dehydrogenase Kinase Isoform 1 antibody
Anti-Pdh Kinase 1 antibody
Anti-PDK1 antibody
Anti-PDHK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance