Anti-PDK1 antibody [4A11] (STJ98315)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PDK1 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Pyruvate dehydrogenase kinase isozyme 1, mitochondrial for use in WB, IHC, IF, FC and ELISA in Human, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/FC/ELISA |
| Host | Mouse |
| Reactivity | Human/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4A11 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDK1 |
| Gene ID | 5163 |
| Uniprot ID | PDK1_HUMAN |
| Immunogen | Purified recombinant fragment of human PDK1 expressed in E. Coli. |
| Specificity | PDK1 Monoclonal Antibody detects endogenous levels of PDK1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect. Phosphorylated under hypoxic conditions at Thr-338 by phosphoglycerate kinase PGK1 which has an activating effect. |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. |
| Protein Name | Pyruvate DehydrogenaseAcetyl-Transferring Kinase Isozyme 1 - MitochondrialPyruvate Dehydrogenase Kinase Isoform 1Pdh Kinase 1 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase antibodyAnti-Acetyl-Transferring Kinase Isozyme 1 - Mitochondrial antibodyAnti-Pyruvate Dehydrogenase Kinase Isoform 1 antibodyAnti-Pdh Kinase 1 antibodyAnti-PDK1 antibodyAnti-PDHK1 antibody |
Information sourced from Uniprot.org