Metabolism | Research Area | St John's Laboratory

HepG2 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Glypican-3 (ABT180) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 58kDaObserved band size: 58kDa

Anti-GPC3 antibody (400-500 aa) [ABT180] (STJ197235)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Western blot analysis of HepG2 cells using Glypican-3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GPC3 antibody (461-510 aa) (STJ96571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using Glypican 3 (GPC3) antibody (STJ11104890) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPC3 antibody (480-580 aa) [S4890RM] (STJ11104890)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using Glypican 3 (GPC3) (GPC3) antibody (STJ26187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GPC3 antibody (537-554) (STJ26187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human hepatocellular carcinoma stained with anti-GPC3 antibody using peroxidase-conjugate and DAB chromogen. Note membranous and granular cytoplasmic staining of tumor cells.

Anti-GPC3 antibody (C-term 70aa) [1G12] (STJ180114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £525.50

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Anti-GPC3 antibody (Met1-His559) [1A080] (STJA0021918)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-GPC3 antibody [SAA0134] (STJA0027025)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-GPC3 antibody [SAA0134] {APC} (STJA0029462)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Anti-GPC3 antibody [SAA0134] {FITC} (STJA0028496)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-GPC3 antibody [SAA0134] {PerCP} (STJA0030428)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£292.50 - £440.50

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Anti-GPC3 antibody [SAA0134] {PE} (STJA0031394)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Anti-GPC3 antibody [SAA2001] (STJA0020548)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£341.50 - £1,447.50

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Anti-GPC3 antibody [YP7] (STJA0020417)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human/Mouse

£341.50 - £1,447.50

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Anti-GPC3 antibody [YP7] (STJA0021855)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-GPC3 nanobody [HN3] (STJN000541)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/Neut

Reactivity :

Human

£378.50 - £1,632.50

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Anti-GPC3/Glypican 3 nanobody [SAA1340] (STJN000233)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of various lysates using GPC4 Rabbit polyclonal antibody (STJ114671) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPC4 antibody (357-556) (STJ114671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-GPC4 antibody (STJA0006930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-GPC4 antibody [SAA1528] (STJA0019956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of K562 cells using Glypican-5 Polyclonal Antibody

Anti-GPC5 antibody (Internal) (STJ93299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using GPC6 antibody (STJ23838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GPC6 antibody (320-530) (STJ23838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-GPCPD1 antibody (STJA0004803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-GPCPD1 antibody (STJA0004804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Western blot analysis of extracts of various cell lines, using GPD1 antibody (STJ28283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GPD1 antibody (1-349) (STJ28283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GPD1 antibody (210-290 aa) (STJ190307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-GPD1L antibody (STJA0007745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using GPD2 Rabbit polyclonal antibody (STJ118806) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GPD2 antibody (25-325) (STJ118806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using GPD2 antibody (STJ28694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GPD2 antibody (25-325) (STJ28694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GPD2 antibody (310-360) (STJA0006575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-GPD2 antibody [SRM13415] (STJ11108145)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£0.00

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Western blot analysis of extracts of various cell lines, using Gephyrin Rabbit monoclonal antibody (STJ11102140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GPHN antibody (1-100) [S0MR] (STJ11102140)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Gephyrin Rabbit pAb (STJ111307) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-GPHN antibody (520-769) (STJ111307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GPHN antibody (STJ190831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-GPHN/Gephyrin antibody [SAA0498] (STJA0019341)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-GPHN/Gephyrin nanobody [SAA1333] (STJN000330)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of extracts from wild type (WT) and glucose-6-phosphateisomerase knockdown (KD) 293T (KD) cells, using glucose-6-phosphateisomerase antibody (STJ11102070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GPI antibody (1-100) [S0MR] (STJ11102070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using glucose-6-phosphate isomerase Rabbit pAb (STJ28996) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GPI antibody (1-280) (STJ28996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using GPI monoclonal antibody against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).

Anti-GPI antibody [1B7D7] (STJ98114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-GPI antibody [SRM15006] (STJ11109736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GPIHBP1 antibody (1-51 aa) (STJ195681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of RAW264.7 cells using GPS2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-GPS2 antibody (11-60 aa) (STJ93406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of mouse tissue with Anti-GPS2 primary antibody (STJ501283) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-GPS2 antibody (250-300) (STJ501283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-GPS2 antibody (250-300) {Biotin} (STJ501284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-GPS2 antibody (250-300) {FITC} (STJ501285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using GPT antibody (STJ23848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPT antibody (1-190) (STJ23848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GPT antibody (STJA0007107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of 293T cells using GPX1 Rabbit polyclonal antibody (STJ23851) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GPX1 antibody (100-200) (STJ23851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of Rat liver, using GPX1 antibody (STJ11104817) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPX1 antibody (100-203) [S4817RM] (STJ11104817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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