Metabolism Of Proteins | Research Area | St John's Laboratory

Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g HTRA2 antibody (STJ117077). Western blot was performed from the immunoprecipitate using HTRA2 antibody (STJ117077) at a dilution of 1:1000.

Anti-HTRA2 antibody (334-458) (STJ117077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot detection of HtrA2/Omi in CHO-K1, MEF and 3T3 cell lysates using HtrA2/Omi mouse mAb (dilution 1:1000).Predicted band size:49kDa.Observed band size:36kDa.

Anti-HTRA2 antibody [1H8-C12-F8] (STJ99318)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Hamster

€180.00 - €435.00

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Western blot detection of HtrA2/Omi in 293T cell lysates using HtrA2/Omi mouse mAb (dilution 1:500).Predicted band size:49kDa.Observed band size:36kDa.

Anti-HTRA2 antibody [8G11-4B8-4F10-G5] (STJ99217)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€180.00 - €435.00

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Anti-Human Tenascin-C antibody [EBS-O-166] (STJ16101005)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human

€359.38 - €1,655.63

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Anti-Hydroxy-HIF1A-P564 antibody (STJ11106685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various cells using Amylin Polyclonal Antibody diluted at 1：1000

Anti-IAPP antibody (1-50 aa) (STJ91590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of 293F-IAPP, using IAPP rabbit polyclonal antibody (STJ24112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IAPP antibody (1-89) (STJ24112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using IARS antibody (STJ112228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IARS antibody (1158-1262) (STJ112228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-IARS1 antibody (STJA0007923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using IARS2 Rabbit polyclonal antibody (STJ118306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IARS2 antibody (663-1012) (STJ118306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using IARS2 Polyclonal Antibody diluted at 1：2000

Anti-IARS2 antibody (71-120 aa) (STJ93632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded rat testis using ICMT Rabbit polyclonal antibody (STJ112331) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ICMT antibody (175-284) (STJ112331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry of mouse kidney with Anti-ICMT primary antibody (STJ501380) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-ICMT antibody (230-284) (STJ501380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-ICMT antibody (230-284) {Biotin} (STJ501381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-ICMT antibody (230-284) {FITC} (STJ501382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-ICMT antibody (STJA0009334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using ICT1 Rabbit polyclonal antibody (STJ113195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ICT1 antibody (30-206) (STJ113195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) Rabbit monoclonal antibody (STJ11101287) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDE antibody (1-100) [S7MR] (STJ11101287)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of A549 cells using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) antibody (STJ24120). Blue: DAPI for nuclear staining.

Anti-IDE antibody (1-250) (STJ24120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Insulin Degrading Enzyme/IDE antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-IDE antibody (56-69 aa) [R07-6D3] (STJA0012660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

€330.63

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Hamster

€60.00 - €315.00

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Western blot analysis of Jurkat, using IDH2 antibody (STJ29270) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IDH2 antibody (193-452) (STJ29270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-IDH2 antibody (STJ193148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U-2 OS cells using IDH3A Rabbit polyclonal antibody (STJ116856) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IDH3A antibody (28-366) (STJ116856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of MCF-7, HT29, mouse brain, mouse heart, mouse colon, mouse kidney, mouse skeletal muscle cells using IDH3A Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH3A antibody (Internal) (STJ96683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of THP-1 treated with LPS (32h) and THP-1 cells using IFIH1 Rabbit polyclonal antibody (STJ115604) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IFIH1 antibody (1-1025) (STJ115604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-IFIH1 antibody (1-205 aa) (STJ11106845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from THP-1 cells using IFIH1 Rabbit polyclonal antibody (STJ11105510) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IFIH1 antibody (277-490) (STJ11105510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various cells using MDA5 Polyclonal Antibody

Anti-IFIH1 antibody (976-1025 aa) (STJ94052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-IFIH1 antibody (STJA0010010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human pancreas. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IGF-I antibody (100-149 aa) (STJ93653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using IGF1 antibody (STJ113891) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IGF1 antibody (50-150) (STJ113891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of IGF1 in paraffin-embedded human lung cancer using IGF1 Rabbit polyclonal antibody (STJ24135) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IGF1 antibody (50-150) (STJ24135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various lysates using IGF1 Rabbit polyclonal antibody (STJ11105441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGF1 antibody (65-165) (STJ11105441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human colon using IGF1 rabbit monoclonal antibody (STJ11101257) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-IGF1 antibody (70-150) [S7MR] (STJ11101257)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Anti-IGF1 antibody (STJA0010178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Human kidney tissue was stained with Anti-IGF-2 (ABT187) Antibody

Anti-IGF2 antibody (1-100 aa) [PT1543] (STJ196899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Immunofluorescence analysis of HepG2 cells using IGF2 Rabbit polyclonal antibody (STJ24139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IGF2 antibody (25-180) (STJ24139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IGF2 antibody (25-40 aa) (STJ191865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Pig

€60.00 - €315.00

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Immunofluorescence analysis of PC-12 cells using IGF2 Rabbit polyclonal antibody (STJ11105485) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IGF2 antibody (70-180) (STJ11105485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of CoLo cells using ALS Polyclonal Antibody

Anti-IGFALS antibody (303-352 aa) (STJ91566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using IGFALS antibody (STJ112261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IGFALS antibody (396-605) (STJ112261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ113709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (150-259) (STJ113709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of extracts of Mouse liver, using IGFBP1 antibody (STJ11101385) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IGFBP1 antibody (160-259) [S5MR] (STJ11101385)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ24145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (81-259) (STJ24145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of IGFBP1 in Human placenta lysates using IGFBP1 antibody.

Anti-IGFBP1 antibody (88-100 aa) [R06-6J8] (STJA0011477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€308.13

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Western blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IGFBP1 antibody (91-140 aa) (STJ97262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using IGFBP2 antibody (STJ24147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IGFBP2 antibody (100-200) (STJ24147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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