Anti-eIF2B5 antibody [R05-6P8] (STJA0036840)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human eIF2B epsilon
General Information
| Short Description | Rabbit monoclonal anti-eIF2B5 for use in WB, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-6P8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EIF2B5 |
| Gene ID | 8893 |
| Uniprot ID | EI2BE_HUMAN |
| Immunogen | A synthesized peptide derived from human eIF2B epsilon |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4. |
| Function | Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. |
| Protein Name | Translation Initiation Factor Eif2b Subunit EpsilonEif2b Gdp-Gtp Exchange Factor Subunit Epsilon |
| Database Links | Reactome: R-HSA-72731 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Translation Initiation Factor Eif2b Subunit Epsilon antibodyAnti-Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon antibodyAnti-EIF2B5 antibodyAnti-EIF2BE antibody |
Information sourced from Uniprot.org