Anti-EIF1AX antibody (10-144) (STJ27713)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF1AX (10-144) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IHC-P:1:100-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF1AX |
| Gene ID | 1964 |
| Uniprot ID | IF1AX_HUMAN |
| Immunogen Region | 10-144 |
| Immunogen Sequence | KNRRRGKNENESEKRELVFK EDGQEYAQVIKMLGNGRLEA MCFDGVKRLCHIRGKLRKKV WINTSDIILVGLRDYQDNKA DVILKYNADEARSLKAYGEL PEHAKINETDTFGPGDDDEI QFDDIGDDDEDIDDI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1). |
Additional Info
| Function | Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1. |
| Protein Name | Eukaryotic Translation Initiation Factor 1a - X-ChromosomalEif-1a X IsoformEif1a X IsoformEukaryotic Translation Initiation Factor 4cEif-4c |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 1a - X-Chromosomal antibodyAnti-Eif-1a X Isoform antibodyAnti-Eif1a X Isoform antibodyAnti-Eukaryotic Translation Initiation Factor 4c antibodyAnti-Eif-4c antibodyAnti-EIF1AX antibodyAnti-EIF1A antibodyAnti-EIF4C antibody |
Information sourced from Uniprot.org