• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) Rabbit polyclonal antibody (STJ112301) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) Rabbit polyclonal antibody (STJ112301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B5 antibody (442-721) (STJ112301)

SKU:
STJ112301

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF2B5 (442-721) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B5
Gene ID: 8893
Uniprot ID: EI2BE_HUMAN
Immunogen Region: 442-721
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human eIF2B epsilon (eIF2B epsilon (EIF2B5) ) (NP_003898.2).
Immunogen Sequence: TLPEGSVISLHPPDAEEDED DGEFSDDSGADQEKDKVKMK GYNPAEVGAAGKGYLWKAAG MNMEEEEELQQNLWGLKINM EEESESESEQSMDSEEPDSR GGSPQMDDIKVFQNEVLGTL QRGKEENISCDNLVLEINSL KYAYNISLKEVMQVLSHVVL EFPLQQMDSPLDSSRYCALL LPLLKAWSPVFRNYIKRAAD HLEALAAIEDFFLEHEALGI SMAKVLMAFYQLEILAEET
Post Translational Modifications Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4.
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Protein Name Translation Initiation Factor Eif-2b Subunit Epsilon
Eif-2b Gdp-Gtp Exchange Factor Subunit Epsilon
Database Links Reactome: R-HSA-72731
Alternative Antibody Names Anti-Translation Initiation Factor Eif-2b Subunit Epsilon antibody
Anti-Eif-2b Gdp-Gtp Exchange Factor Subunit Epsilon antibody
Anti-EIF2B5 antibody
Anti-EIF2BE antibody

Information sourced from Uniprot.org

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