Metabolism Of Proteins | Research Area | St John's Laboratory

Immunofluorescence analysis of HepG2 cells using IGF2 Rabbit polyclonal antibody (STJ24139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IGF2 antibody (25-180) (STJ24139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IGF2 antibody (25-40 aa) (STJ191865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of PC-12 cells using IGF2 Rabbit polyclonal antibody (STJ11105485) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IGF2 antibody (70-180) (STJ11105485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of CoLo cells using ALS Polyclonal Antibody

Anti-IGFALS antibody (303-352 aa) (STJ91566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using IGFALS antibody (STJ112261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IGFALS antibody (396-605) (STJ112261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ113709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (150-259) (STJ113709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of Mouse liver, using IGFBP1 antibody (STJ11101385) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IGFBP1 antibody (160-259) [S5MR] (STJ11101385)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Western blot analysis of extracts of HepG2 cells, using IGFBP1 antibody (STJ24145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IGFBP1 antibody (81-259) (STJ24145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of IGFBP1 in Human placenta lysates using IGFBP1 antibody.

Anti-IGFBP1 antibody (88-100 aa) [R06-6J8] (STJA0011477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£246.50

Choose Options

Western blot analysis of MCF7 cells using IGFBP1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IGFBP1 antibody (91-140 aa) (STJ97262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using IGFBP2 antibody (STJ24147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IGFBP2 antibody (100-200) (STJ24147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis using IGFBP2 monoclonal antibody against truncated IGFBP2-His recombinant protein (1) and truncated IGFBP2 (aa40-328) -hIgGFc transfected CHO-K1 cell lysate (2).

Anti-IGFBP2 antibody (180-328 aa) [1F6F6] (STJ98157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis of extracts of various cell lines, using IGFBP3 antibody (STJ118493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP3 antibody (100-200) (STJ118493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using IGFBP3 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-IGFBP3 antibody (151-200 aa) (STJ93651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IGFBP4 antibody (100-180 aa) (STJ190312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using IGFBP4 Rabbit polyclonal antibody (STJ117262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IGFBP4 antibody (178-258) (STJ117262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of L929 cells using IGFBP4 Rabbit polyclonal antibody (STJ26194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IGFBP4 antibody (22-258) (STJ26194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts from wild type (WT) and IGFBP5 knockout (KO) 293T cells, using IGFBP5 antibody (STJ11104514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP5 antibody (21-272) (STJ11104514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of mouse kidney, using IGFBP5 antibody (STJ114325) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IGFBP5 antibody (68-167) (STJ114325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using IGFBP5 Rabbit polyclonal antibody (STJ119343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP5 antibody (68-167) (STJ119343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-IGFBP5 antibody (90-170 aa) (STJ190313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of K562 cells using IGFBP6 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IGFBP6 antibody (101-150 aa) (STJ96726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using IGFBP6 Rabbit pAb (STJ112233) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-IGFBP6 antibody (28-240) (STJ112233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IBP7 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IGFBP7 antibody (191-240 aa) (STJ93652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using IGFBP7 Rabbit polyclonal antibody (STJ114990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IGFBP7 antibody (27-282) (STJ114990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Confocal imaging of MCF7 cells using IGFBP7 Rabbit monoclonal antibody (STJ11102117, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-IGFBP7 antibody (50-150) [S7MR] (STJ11102117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-IgM-k antibody [EBS-CD-019] (STJ16102586)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£307.50 - £1,449.50

Choose Options

Western blot analysis of extracts of various cell lines, using IKK Epsilon rabbit monoclonal antibody (STJ11101875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IKBKE antibody (1-100) [S5MR] (STJ11101875)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis using IKK Epsilon monoclonal antibody against truncated IKK Epsilon recombinant protein (1) and full-length IKK Epsilon (aa1-716) -hIgGFc transfected COS7 cell lysate (2).

Anti-IKBKE antibody (1-257 aa) [6B4B5] (STJ98166)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse brain using IKK Epsilon antibody (STJ116028) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IKBKE antibody (495-716) (STJ116028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of THP-1 cells using IKK Epsilon Rabbit polyclonal antibody (STJ24153) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IKBKE antibody (497-716) (STJ24153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunocytochemistry analysis of IKK gamma (green) in Hela using IKK gamma antibody, and DAPI (blue)

Anti-IKBKG antibody (1-419 aa) [R06-5G4] (STJA0011488)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of A549 cells using 3 Mu g IKK Gamma antibody (STJ114410). Western blot was performed from the immunoprecipitate using IKK Gamma antibody (STJ114410) at a dilution of 1:1000.

Anti-IKBKG antibody (1-419) (STJ114410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of U-2 OS cells using IKK Gamma Rabbit polyclonal antibody (STJ24154) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IKBKG antibody (250-390) (STJ24154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using IKK Gamma Polyclonal Antibody diluted at 1：1000

Anti-IKBKG antibody (51-100 aa) (STJ93672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-IKBKG antibody (C-Term) (STJA0005691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

Choose Options

Anti-IKBKG antibody (N-Term) (STJA0005690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

Choose Options

Anti-IL-6 antibody [2707] (STJ400294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£507.50 - £659.50

Choose Options

Anti-IL-6 antibody [MP5-20F3] (STJ16100654)

Clonality :

Monoclonal

Host :

Rat

Applications :

FUNC/ELISA/IP

Reactivity :

Mouse

£460.50

Choose Options

Anti-IL33 antibody (1-270) (STJ114478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

Choose Options

Anti-IL33 antibody (109-270 aa) [SRM] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates using IL33 Rabbit pAb (STJ110395) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-IL33 antibody (110-190) (STJ110395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of HeLa cells using IL-33 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL33 antibody (121-170 aa) (STJ96852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using IL6 Rabbit pAb (STJ111153) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-IL6 antibody (1-100) (STJ111153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from HUVEC cells, using IL6 Rabbit monoclonal antibody (STJ11104756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IL6 antibody (1-212) [S4756RM] (STJ11104756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, IL-6 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL6 antibody (131-180 aa) (STJ96713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit polyclonal antibody (STJ110936) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6 antibody (30-212) (STJ110936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with IL6 using IL6 Rabbit polyclonal antibody (STJ113711) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IL6 antibody (30-212) (STJ113711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options