Anti-EEF1A1 antibody (325-338 aa) [R04-3H4] (STJA0012354)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human eEF1A1/EF-Tu
General Information
| Short Description | Rabbit monoclonal anti-Elongation Factor 1A1 (325-338 aa) for use in WB, IHC-F, IHC-P, ICC and IF in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-3H4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EEF1A1 |
| Gene ID | 1915 |
| Uniprot ID | EF1A1_HUMAN |
| Immunogen | A synthetic peptide of human eEF1A1/EF-Tu |
| Immunogen Region | 325-338 aa |
Additional Info
| Post Translational Modifications | ISGylated. Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2. Phosphorylation by TGFBR1 inhibits translation elongation. Trimethylated at Lys-79 by EEF1AKMT1. Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation. Trimethylated at Lys-318 by EEF1AKMT2. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at Gly-2 by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant. Ubiquitinated at Lys-385 by RNF14 in response to ribosome collisions (ribosome stalling), leading to its degradation by the proteasome and rescue of stalled ribosomes. |
| Function | Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection. |
| Protein Name | Elongation Factor 1-Alpha 1Ef-1-Alpha-1Elongation Factor TuEf-TuEukaryotic Elongation Factor 1 A-1Eef1a-1Leukocyte Receptor Cluster Member 7 |
| Database Links | Reactome: R-HSA-156842Reactome: R-HSA-156902Reactome: R-HSA-3371511Reactome: R-HSA-6798695Reactome: R-HSA-8876725Reactome: R-HSA-9613829Reactome: R-HSA-9735869 |
| Cellular Localisation | CytoplasmNucleusNucleolusCell MembraneColocalizes With Dlc1 At Actin-Rich Regions In The Cell PeripheryTranslocates Together With Zpr1 From The Cytoplasm To The Nucleus And Nucleolus After Treatment With MitogensLocalization At The Cell Membrane Depends On Eef1a1 Phosphorylation Status And The Presence Of Ppp1r16b |
| Alternative Antibody Names | Anti-Elongation Factor 1-Alpha 1 antibodyAnti-Ef-1-Alpha-1 antibodyAnti-Elongation Factor Tu antibodyAnti-Ef-Tu antibodyAnti-Eukaryotic Elongation Factor 1 A-1 antibodyAnti-Eef1a-1 antibodyAnti-Leukocyte Receptor Cluster Member 7 antibodyAnti-EEF1A1 antibodyAnti-EEF1A antibodyAnti-EF1A antibodyAnti-LENG7 antibody |
Information sourced from Uniprot.org