Anti-EEF1D antibody (1-160) (STJ23477)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EEF1D (1-160) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF1D |
| Gene ID | 1936 |
| Uniprot ID | EF1D_HUMAN |
| Immunogen Region | 1-160 |
| Immunogen Sequence | MATNFLAHEKIWFDKFKYDD AERRFYEQMNGPVAGASRQE NGASVILRDIARARENIQKS LAGSSGPGASSGTSGDHGEL VVRIASLEVENQSLRGVVQE LQQAISKLEARLNVLEKSSP GHRATAPQTQHVSPMRQVEP PAKKPATPAEDDEDDDIDLF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1). |
Additional Info
| Tissue Specificity | Isoform 2 is specifically expressed in brain, cerebellum and testis. |
| Function | Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). |
| Protein Name | Elongation Factor 1-DeltaEf-1-DeltaAntigen Ny-Co-4 |
| Database Links | Reactome: R-HSA-156842 |
| Cellular Localisation | Isoform 2: Nucleus |
| Alternative Antibody Names | Anti-Elongation Factor 1-Delta antibodyAnti-Ef-1-Delta antibodyAnti-Antigen Ny-Co-4 antibodyAnti-EEF1D antibodyAnti-EF1D antibody |
Information sourced from Uniprot.org