Metabolism Of Proteins | Research Area | St John's Laboratory

Immunofluorescence analysis of U2OS cells using Histone H2AX Rabbit polyclonal antibody (STJ113704) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-H2AFX antibody (50-143) (STJ113704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Histone H2A.X Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H2AX antibody (94-143 aa) (STJ93520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/CoIP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HELA Cell Lysate using antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-H2AZ1 antibody (1-30 aa) (STJ98947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2AZ1 antibody (1-80 aa) (STJ192899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-H2B1C antibody (STJ193068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Histone H2B using Histone H2B (testis specific) Rabbit polyclonal antibody (STJ11104985) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-H2BC1 antibody (STJ11104985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-H2BC11 antibody (58-108 aa) (STJ197695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-H2BC12L antibody (10-59 aa) (STJ93522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2BC13 antibody (34-84 aa) (STJ195934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H2BC21 antibody (18-68 aa) (STJ194032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-H2BC3 antibody (C-Term) (STJA0005436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£365.50

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Anti-H2BC3 antibody (N-Term) (STJA0005437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunofluorescence analysis of Hela cell. 1, Brp44L Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H2BC3 antibody [Mix] (STJ97064)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Histone H3.3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H3-3A/H3-3B antibody (16-65 aa) (STJ93535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12 antibody (STJA0010015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using H3F3B antibody (STJ112258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-H3F3B antibody (1-136) (STJ112258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of MCF7, using Lactic acid-Histone H4-K5 rabbit polyclonal antibody (STJ11101520) at 1:1000 dilution. MCF7 cells were treated by Sodium lactate (100mM) for 24h. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-H4K5la antibody (STJ11101520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using HADH antibody (STJ23909) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HADH antibody (1-314) (STJ23909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HADH antibody (STJA0006880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1：2000

Anti-HARS1 antibody (460-509 aa) (STJ93504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HARS2 antibody (STJ110095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HARS2 antibody (217-506) (STJ110095)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HARS2 antibody (STJA0007722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HCFC1 antibody (STJ119209) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-HCFC1 antibody (1-250) (STJ119209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HCFC1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HCFC1 antibody (131-180 aa) (STJ93473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-hCG antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 13 (17) kDaObserved band size: 13 kDa

Anti-hCG antibody (25-100 aa) [ABT-HCG] (STJ196767)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-hCG antibody [SM5034] (STJA0005034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HDAC1 (PTR1326) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 55kDaObserved band size: 62kDa

Anti-HDAC1 antibody (300-482) [PTR1326] (STJA0006279)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300ug extracts of 293T cells using 0. 5ug HDAC1 antibody (STJ11101705). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ11101705) at a dilition of 1:1000.

Anti-HDAC1 antibody (350-450) [S5MR] (STJ11101705)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using HDAC1 Polyclonal Antibody diluted at 1：500

Anti-HDAC1 antibody (387-436 aa) (STJ93477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells, using 3 Mu g [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ23928) at a dilution of 1:1000.

Anti-HDAC1 antibody (393-482) (STJ23928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Histone deacetylase 1 Polyclonal Antibody diluted at 1：1000

Anti-HDAC1 antibody (433-482 aa) (STJ93510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HDAC1 antibody (Internal Region) (STJ13101199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, HDAC1 Mouse monoclonal antibody (4E1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-HDAC1 antibody [4E1] (STJ97722)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC2 antibody (STJ11101736). Western blot was performed from the immunoprecipitate using HDAC2 antibody (STJ11101736) at a dilution of 1:1000.

Anti-HDAC2 antibody (1-100) [S6MR] (STJ11101736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using HDAC2 Polyclonal Antibody diluted at 1：2000

Anti-HDAC2 antibody (360-409 aa) (STJ93478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g HDAC2 antibody (STJ23929). Western blot was performed from the immunoprecipitate using HDAC2 antibody (STJ23929) at a dilution of 1:1000.

Anti-HDAC2 antibody (390-488) (STJ23929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from H460 cells, using HDAC2 Rabbit pAb (STJ111039) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HDAC2 antibody (429-488) (STJ111039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of mouse liver, using HDAC2 antibody (STJ115577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC2 antibody (429-488) (STJ115577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using HDAC3 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , C6 (3) and COS (4) cell lysate.

Anti-HDAC3 antibody (224-428 aa) [7G6C5] (STJ98129)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-HDAC3 antibody (299-428) (STJ23930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (STJ114416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HDAC3 antibody (329-428) (STJ114416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HDAC3 Rabbit polyclonal antibody (STJ118890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC3 antibody (329-428) (STJ118890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g HDAC3 Rabbit monoclonal antibody (STJ11101684). Western blot was performed from the immunoprecipitate using HDAC3 Rabbit monoclonal antibody (STJ11101684) at a dilution of 1:1000.

Anti-HDAC3 antibody (329-428) [S4MR] (STJ11101684)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using HDAC3 Polyclonal Antibody diluted at 1：1000

Anti-HDAC3 antibody (379-428 aa) (STJ93479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using SMAP45 Polyclonal Antibody diluted at 1：2000

Anti-HDAC3 antibody (379-428 aa) (STJ95704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of HDAC3 in (1) HeLa lysates; (2) NIH/3T3 lysates using HDAC3 antibody.

Anti-HDAC3 antibody (400-428 aa) [R07-8E9] (STJA0014653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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