• Western blot analysis of extracts of various cell lines, using DCAF7 antibody (STJ28870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using DCAF7 rabbit polyclonal antibody (STJ28870) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DCAF7 antibody (1-342) (STJ28870)

SKU:
STJ28870

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCAF7 (1-342) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCAF7
Gene ID: 10238
Uniprot ID: DCAF7_HUMAN
Immunogen Region: 1-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
Immunogen Sequence: MSLHGKRKEIYKYEAPWTVY AMNWSVRPDKRFRLALGSFV EEYNNKVQLVGLDEESSEFI CRNTFDHPYPTTKLMWIPDT KGVYPDLLATSGDYLRVWRV GETETRLECLLNNNKNSDFC APLTSFDWNEVDPYLLGTSS IDTTCTIWGLETGQVLGRVN LVSGHVKTQLIAHDKEVYDI AFSRAGGGRDMFASVGADGS VRMFDLRHLEHSTIIYEDPQ HHPLLRLCWNKQDPNYLAT
Function Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Protein Name Ddb1- And Cul4-Associated Factor 7
Wd Repeat-Containing Protein 68
Wd Repeat-Containing Protein An11 Homolog
Database Links Reactome: R-HSA-390471
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Nucleus
Overexpression Of Diahp1 Or Active Rhoa Causes Translocation From The Nucleus To Cytoplasm
Alternative Antibody Names Anti-Ddb1- And Cul4-Associated Factor 7 antibody
Anti-Wd Repeat-Containing Protein 68 antibody
Anti-Wd Repeat-Containing Protein An11 Homolog antibody
Anti-DCAF7 antibody
Anti-HAN11 antibody
Anti-WDR68 antibody

Information sourced from Uniprot.org

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