Anti-DDOST antibody (40-300) (STJ111545)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DDOST (40-300) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DDOST |
| Gene ID | 1650 |
| Uniprot ID | OST48_HUMAN |
| Immunogen Region | 40-300 |
| Immunogen Sequence | VCASGPRTLVLLDNLNVRET HSLFFRSLKDRGFELTFKTA DDPSLSLIKYGEFLYDNLII FSPSVEDFGGNINVETISAF IDGGGSVLVAASSDIGDPLR ELGSECGIEFDEEKTAVIDH HNYDISDLGQHTLIVADTEN LLKAPTIVGKSSLNPILFRG VGMVADPDNPLVLDILTGSS TSYSFFPDKPITQYPHAVGK NTLLIAGLQARNNARVIFSG SLDFFSDSFFNSAVQKAAP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human DDOST (NP_005207.2). |
Additional Info
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Required for the assembly of both SST3A- and SS3B-containing OST complexes. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 48 Kda SubunitDdost 48 Kda SubunitOligosaccharyl Transferase 48 Kda Subunit |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-6798695Reactome: R-HSA-879415Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 48 Kda Subunit antibodyAnti-Ddost 48 Kda Subunit antibodyAnti-Oligosaccharyl Transferase 48 Kda Subunit antibodyAnti-DDOST antibodyAnti-KIAA0115 antibodyAnti-OST48 antibodyAnti-OK antibodyAnti-SW-cl.45 antibody |
Information sourced from Uniprot.org