• Western blot analysis of various lysates, using DCTN2 Rabbit polyclonal antibody (STJ117041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DCTN2 antibody (280-401) (STJ117041)

SKU:
STJ117041

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCTN2 (280-401) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCTN2
Gene ID: 10540
Uniprot ID: DCTN2_HUMAN
Immunogen Region: 280-401
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-401 of human DCTN2 (NP_006391.1).
Immunogen Sequence: VLDQVEARLQSVLGKVNEIA KHKASVEDADTQSKVHQLYE TIQRWSPIASTLPELVQRLV TIKQLHEQAMQFGQLLTHLD TTQQMIANSLKDNTTLLTQV QTTMRENLATVEGNFASIDE RM
Function Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length. Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Protein Name Dynactin Subunit 2
50 Kda Dynein-Associated Polypeptide
Dynactin Complex 50 Kda Subunit
Dctn-50
P50 Dynamitin
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-2565942
Reactome: R-HSA-3371497
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Dynactin Subunit 2 antibody
Anti-50 Kda Dynein-Associated Polypeptide antibody
Anti-Dynactin Complex 50 Kda Subunit antibody
Anti-Dctn-50 antibody
Anti-P50 Dynamitin antibody
Anti-DCTN2 antibody
Anti-DCTN50 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance