Metabolism Of Proteins | Research Area | St John's Laboratory

Anti-TOMM20 antibody (20-145) [S5MR] (STJ11101675)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Anti-TOMM20 antibody (25-145aa) [AT1B2] (STJA0041855)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

$302.91 - $458.85

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Anti-TOMM20 antibody [PT0287R] (STJA0039674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-TOMM20 antibody [R03-7F6] (STJA0034939)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Hamster

$184.23 - $388.47

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Western blot analysis of extracts of various cell lines, using TOM70 rabbit polyclonal antibody antibody (STJ25915) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TOMM70 antibody (245-608) (STJ25915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using TOM70 antibody (STJ11104231) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TOMM70 antibody (245-608) [S4231RM] (STJ11104231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various lysates, using TOMM70 antibody (STJ11104820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TOMM70 antibody (245-608) [S4820RM] (STJ11104820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Anti-TOMM70 antibody (STJA0006746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g DNA topoisomerase I (DNA topoisomerase I (TOP1) ) antibody (STJ114398). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (DNA topoisomerase I (TOP1) ) antibody (STJ114398) at a dilution of 1:1000.

Anti-TOP1 antibody (1-100) (STJ114398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g DNA topoisomerase I (TOP1) antibody (STJ11101411). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (TOP1) antibody (STJ11101411) at a dilution of 1:1000.

Anti-TOP1 antibody (1-100) [S1MR] (STJ11101411)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Immunocytochemistry analysis of Topoisomerase I (green) in Hela using Topoisomerase I antibody, and DAPI (blue)

Anti-TOP1 antibody (2-15 aa) [R02-2H9] (STJA0012067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-TOP1 antibody (Internal) (STJ96062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Topo II Alpha Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-TOP2A antibody (1-50 aa) (STJ96066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (TOP2A) antibody (STJ118868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TOP2A antibody (1100-1200) (STJ118868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot with Anti-TOP2A Antibody (STJ503357) with TOPO2A peptide. Antibody at 1:500 dilution in buffer.

Anti-TOP2A antibody (1220-1270) (STJ503357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-TOP2A antibody (1220-1270) {Biotin} (STJ503358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-TOP2A antibody (1220-1270) {FITC} (STJ503359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Immunofluorescence analysis of U-2 OS cells using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A) ) Rabbit polyclonal antibody (STJ11103810) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TOP2A antibody (1314-1503) (STJ11103810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Topoisomerase IIα (ABT272) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 174kDaObserved band size: 174kDa

Anti-TOP2A antibody (1400-1531 aa) [ABT272] (STJ197194)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Immunohistochemistry analysis of Topo II Alpha in paraffin-embedded human cervix cancer tissue using Topo II Alpha Rabbit polyclonal antibody (STJ11105362) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-TOP2A antibody (1431-1531) (STJ11105362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using DNA DNA topoisomerase II alpha (TOP2A) Rabbit monoclonal antibody (STJ11102067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TOP2A antibody (1432-1531) [S7MR] (STJ11102067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Anti-TOP2A antibody [IHC112] (STJA0042275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

$215.97 - $623.07

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Anti-TOP2A antibody [IHC432] (STJA0042066)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

$204.93 - $790.05

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TOP2B antibody (1-50 aa) (STJ96068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-Topoisomerase IIa antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Anti-Topoisomerase II alpha antibody (1431-1531aa) [ZR94] (STJ180481)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$229.77 - $943.23

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Anti-Topoisomerase II alpha antibody [1D2-2H6-7D1] (STJA0033490)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$296.01 - $425.73

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Anti-Topoisomerase II alpha antibody [BP6016] (STJA0037586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$337.41 - $462.99

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Anti-Topoisomerase II alpha antibody [R04-8J9] (STJA0035735)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Topoisomerase II alpha/TOP2A antibody [SRM14910] (STJ11109640)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-TOPORS antibody (STJ191816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunocytochemistry analysis of p53 (green) in MCF-7 using p53 antibody, and DAPI (blue)

Anti-TP53 antibody (1-101 aa) [R07-6A7] (STJA0011734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit polyclonal antibody (STJ25926). Western blot analysis was performed using p53 Rabbit polyclonal antibody (STJ25926) at 1:3000 dilution.

Anti-TP53 antibody (1-393) (STJ25926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713) at a dilution of 1:1000.

Anti-TP53 antibody (1-393) [S3MR] (STJ11101713)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

$198.03 - $853.53

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Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-TP53 antibody (10-59 aa) (STJ94893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunoprecipitation analysis of 200 Mu g extracts of U-251MG cells using 3 Mu g p53 antibody (STJ28328). Western blot was performed from the immunoprecipitate using p53 antibody (STJ28328) at a dilution of 1:1000.

Anti-TP53 antibody (218-390) (STJ28328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$152.49 - $697.59

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Human esophageal squamous cell carcinoma tissue was stained with Anti-P53 (ABT-P53) Antibody

Anti-TP53 antibody (250-393 aa) [ABT-P53] (STJ197083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 (ABT002) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 53kDaObserved band size: 53kDa

Anti-TP53 antibody (250-393 aa) [ABT002] (STJ197180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Anti-P53 antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140047)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

$130.41 - $607.89

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Anti-P53 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140114)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

$130.41 - $607.89

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Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TP53 antibody (283-332 aa) (STJ94905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of various cells using p53 Polyclonal Antibody diluted at 1：1000

Anti-TP53 antibody (301-350 aa) (STJ94894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha (phospho Ser51) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TP53 antibody [6C4] (STJ96954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

$67.62 - $360.18

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Human breast carcinoma infiltrating fat stained with anti-p53 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of carcinoma cells.

Anti-TP53 antibody [DO‐7] (STJ180207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$202.17 - $639.63

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Anti-TP53 antibody [IHC053] (STJA0042019)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

$159.39 - $393.99

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Anti-TP53 antibody [Pab122] (STJ16100763)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Dog/Human/Monkey/Mouse/Rat

$396.75 - $1,827.81

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Western blot analysis of various lysates using p53 Mouse monoclonal antibody (STJ112620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TP53 antibody [S0MM] (STJ112620)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Western blot analysis of lysates from 293T cells using [KO Validated] p53 Rabbit monoclonal antibody (STJ11105127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TP53 antibody [S5127RM] (STJ11105127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$198.03 - $853.53

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Anti-TP53/p53 (R175H mutant) antibody [F2] (STJA0027800)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $395.37

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