• Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g DNA topoisomerase I (TOP1) antibody (STJ11101411). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (TOP1) antibody (STJ11101411) at a dilution of 1:1000.
  • Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit monoclonal antibody (STJ11101411, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of DNA topoisomerase I (TOP1) in paraffin-embedded human thyroid cancer using DNA topoisomerase I (DNA topoisomerase I (TOP1) ) Rabbit monoclonal antibody (STJ11101411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse thymus, using DNA topoisomerase I (DNA topoisomerase I (TOP1) ) Rabbit monoclonal antibody (STJ11101411) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using DNA topoisomerase I (DNA topoisomerase I (TOP1) ) Rabbit monoclonal antibody (STJ11101411) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TOP1 antibody (1-100) [S1MR] (STJ11101411)

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STJ11101411

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TOP1 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP1
Gene ID: 7150
Uniprot ID: TOP1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).
Immunogen Sequence: MSGDHLHNDSQIEADFRLND SHKHKDKHKDREHRHKEHKK EKDREKSKHSNSEHKDSEKK HKEKEKTKHKDGSSEKHKDK HKDRDKEKRKEEKVRASGDA
Tissue Specificity Endothelial cells.
Post Translational Modifications Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.
Protein Name Dna Topoisomerase 1
Dna Topoisomerase I
Database Links Reactome: R-HSA-4615885
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Diffuse Nuclear Localization With Some Enrichment In Nucleoli
On Cpt Treatment
Cleared From Nucleoli Into Nucleoplasm
Sumoylated Forms Found In Both Nucleoplasm And Nucleoli
Alternative Antibody Names Anti-Dna Topoisomerase 1 antibody
Anti-Dna Topoisomerase I antibody
Anti-TOP1 antibody

Information sourced from Uniprot.org

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