Anti-TOP2A antibody (1220-1270) {Biotin} (STJ503358)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-TOP2A (1220-1270) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.54-0.57 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TOP2A |
| Gene ID | 7153 |
| Uniprot ID | TOP2A_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein. |
| Immunogen Region | 1220-1270 |
Additional Info
| Tissue Specificity | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas. |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Protein Name | Dna Topoisomerase 2-AlphaDna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-4615885 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleolus |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibodyAnti-Dna Topoisomerase Ii - Alpha Isozyme antibodyAnti-TOP2A antibodyAnti-TOP2 antibody |
Information sourced from Uniprot.org