Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using Phospho-ATF2-T71 Rabbit pAb (STJ22025) at 1:1000 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ATF2-T71 antibody (STJ22025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (STJ11102621) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-ATF2-T71 antibody [S1MR] (STJ11102621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot phosphorylated ATF2 protein at Thr52 in UACC903 melanoma cells STJA0003584

Anti-Phospho-ATF2-Thr52 antibody (STJA0003584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€586.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr69 or 51) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Thr69 antibody (36-85 aa) (STJ90185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-ATF2-Thr69/71 antibody (STJ196245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ATF2-Thr71 antibody (38-87 aa) (STJ90186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Phospho-ATF2 Thr71 antibody immunocytochemistry U87-MG STJA0034048

Anti-Phospho-ATF2-Thr71 antibody [R07-3E5] (STJA0034048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

€160.20 - €337.80

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Western blot analysis of various cells using Phospho-ATF-2 (T73) Polyclonal Antibody

Anti-Phospho-ATF2-Thr73 antibody (40-89 aa) (STJ90187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using (STJ22028) at 1:1000 dilution. 293T cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATM-S1981 antibody (STJ22028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

€438.60

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Western blot ATM phosphorylation in A431 cells with phosphatase treatment comparison STJA0041054

Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0041054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

€438.60

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ICC staining ATM phosphorylation in A431 cells using anti-ATM Ser-794 antibody STJA0041068

Anti-Phospho-ATM-Ser-794 antibody (STJA0041068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/FC

Reactivity :

Human/Mouse/Rat

€651.00

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Phospho-ATM Ser1981 antibody western blot HEK293 STJA0035784

Anti-Phospho-ATM-Ser1981 antibody [R01-5I8] (STJA0035784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-ATR-Thr1989 antibody (STJ196248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATRIP (Phospho-Ser224) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATRIP-Ser224 antibody (190-239 aa) (STJ90973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot ATRIP protein phosphorylation at Ser239 in mouse testicular lysate STJA0003585

Anti-Phospho-ATRIP-Ser239 antibody (STJA0003585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Bovine/Human/Non-Human Primates/Sheep

€586.20

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Western blot analysis of NIH-3T3 cells using Phospho-ATRIP (S68) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ATRIP-Ser68 antibody (34-83 aa) (STJ90496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATXN3-Ser256 antibody (STJA0009980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AurA (Phospho-Ser342) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKA-Ser342 antibody (311-360 aa) (STJ91174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates, using Phospho-AURKA-T288 Rabbit pAb (STJ110859) at 1:1000 dilution. HeLa were treated by Hydroxyurea (4mM) or treated by Nocodazole (50ng/mL) for 20 hours. HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AURKA-T288 antibody (STJ110859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of 293 cells using Phospho-ARK-1 (T288) Polyclonal Antibody

Anti-Phospho-AURKA-Thr288 antibody (256-305 aa) (STJ90805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Thr232 antibody (198-247 aa) (STJ91089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Tyr12 antibody (1-50 aa) (STJ90920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Bax (Phospho-Ser184) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BAX-Ser184 antibody (143-192 aa) (STJ91241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of A549 cells, using Bax (Phospho-Thr167) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BAX-Thr167 antibody (133-182 aa) (STJ91329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BCL2L11-Ser59 antibody (31-80 aa) (STJ91225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-BCL2L11-Ser69 antibody (STJ194702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-Phospho-BCL2L11-Ser77 antibody (STJ194703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western Blot analysis of 1 Jurkat cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BCL2L11-Thr56/116 antibody (STJA0010071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of MOUSE-HEART cells using Phospho-Bcl-6 (S333) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-BCL6-Ser333 antibody (299-348 aa) (STJ90620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of 1 Raji cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BCL6-Ser343 antibody (STJA0010020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Phospho-beta Catenin antibody western blot 293T lysates STJA0036213

Anti-Phospho-beta Catenin-Ser33/Ser37 antibody [R03-5O3] (STJA0036213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€160.20 - €337.80

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Anti-Phospho-Beta-Catenin-Tyr-142 antibody (STJA0041137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-333 antibody (STJA0041158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-489 antibody (STJA0041156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Beta-Catenin-Tyr-654 antibody (STJA0041163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€651.00

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