Anti-Phospho-BCL2L11-Thr56/116 antibody (STJA0010071)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human Bim (Phospho Thr56/116)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Bcl-2-like protein 11-Thr56/116 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCL2L11 |
| Gene ID | 10018 |
| Uniprot ID | B2L11_HUMAN |
| Immunogen | Synthesized peptide derived from human Bim (Phospho Thr56/116) |
| Specificity | This antibody detects endogenous levels of Bim (Phospho Thr56/116) Rabbit pAb at Human, Mouse, Rat |
Additional Info
| Tissue Specificity | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. |
| Post Translational Modifications | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Protein Name | Bcl-2-Like Protein 11Bcl2-L-11Bcl2-Interacting Mediator Of Cell Death |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-6802952Reactome: R-HSA-8862803Reactome: R-HSA-8952158Reactome: R-HSA-9607240Reactome: R-HSA-9614657 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinAssociated With Intracytoplasmic MembranesIsoform Bimel: MitochondrionTranslocates From Microtubules To Mitochondria On Loss Of Cell AdherenceIsoform Biml: MitochondrionIsoform Bims: MitochondrionIsoform Bim-Alpha1: Mitochondrion |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 11 antibodyAnti-Bcl2-L-11 antibodyAnti-Bcl2-Interacting Mediator Of Cell Death antibodyAnti-BCL2L11 antibodyAnti-BIM antibody |
Information sourced from Uniprot.org