Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using MOBP Rabbit polyclonal antibody (STJ119481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-MOBP antibody (1-70) (STJ119481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MOBP antibody (30-110 aa) (STJ190995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using MonoMethyl-H2B-E105 Rabbit polyclonal antibody (STJ11103233) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Mono-Methyl-H2B-E105-E105 antibody (STJ11103233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts from Hela cells, 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H4-Arg3 antibody (STJ97197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ11103476) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Mono-Methyl-Histone H4-K20 antibody (STJ11103476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23970). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-K20 antibody (STJ23970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H4-K5 Rabbit polyclonal antibody (STJ119872) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-K5 antibody (STJ119872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 3T3 mouse-kidney KB K562 Hela 293T lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-Histone H4-Lys21 antibody (21 aa) (STJ98696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H4-Lys79 antibody (STJ97157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ11100898). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-R3 antibody (STJ11100898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of MCF7 cells using Mono-Methyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-RELA-Lys314/Lys315 antibody (STJ90114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T COLO using TP53 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-TP53-Lys370 antibody (STJ97694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using P53 (Mono Methyl Lys370) Mouse mAb diluted at 1:500.

Anti-Mono-Methyl-TP53-Q59 antibody [Q59] (STJ97495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (STJ118448) at 1:1000 dilution.

Anti-Mono-Methyl-UHRF1-K385 antibody (STJ118448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit polyclonal antibody (STJ11100581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Mono-Methyl-YAP1-K342 antibody (STJ11100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H4-R3 Rabbit pAb (STJ11106422) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MonoMethyl-Histone H4-R3 antibody (STJ11106422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-MOV10 antibody (13-63) (STJA0006459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Rat kidney, using MOV10 Rabbit monoclonal antibody (STJ11102335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MOV10 antibody (800-900) [S5MR] (STJ11102335)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using MOV10L1 Polyclonal Antibody diluted at 1：500

Anti-MOV10L1 antibody (318-367) (STJ94184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using MRE11 Rabbit polyclonal antibody (STJ24608) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MRE11 antibody (1-302) (STJ24608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using MRE11 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MRE11 antibody (230-279) (STJ94194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g MRE11 antibody (STJ115961). Western blot was performed from the immunoprecipitate using MRE11 antibody (STJ115961) at a dilution of 1:1000.

Anti-MRE11 antibody (290-485) (STJ115961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using MRE11 rabbit monoclonal antibody (STJ11102020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MRE11 antibody (400-500) [S0MR] (STJ11102020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates using MSH2 Rabbit mAb (STJ11102365) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-MSH2 antibody (1-100) [S5MR] (STJ11102365)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g MSH2 antibody (STJ11104374). Western blot was performed from the immunoprecipitate using MSH2 antibody (STJ11104374) at a dilution of 1:3000.

Anti-MSH2 antibody (1-300) (STJ11104374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunofluorescence analysis of C6 cells using MSH2 Rabbit polyclonal antibody (STJ24611) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MSH2 antibody (1-300) (STJ24611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with anti-MSH-2 antibody using peroxidase-conjugate and DAB chromogen. Note the absence nuclear staining of tumor cells and the presence of nuclear staining of normal mucosa

Anti-MSH2 antibody (327-427aa) [ZR260] (STJ180522)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£182.50 - £722.50

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Western blot analysis of extracts from wild type (WT) and MSH2 knockout (KO) HeLa cells, using MSH2 antibody (STJ11104510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MSH2 antibody (330-583) (STJ11104510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunofluorescence analysis of HUVEC cells, using MSH2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MSH2 antibody (541-590) (STJ94261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Human colon carcinoma with msH2 expression tissue was stained with Anti-MSH2 (ABT-MSH2) Antibody

Anti-MSH2 antibody (600-700 aa) [ABT-MSH2] (STJ197034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis using MSH2 monoclonal antibody against HeLa (1) , A549 (2) , A431 (3) and HEK293 (4) cell lysate.

Anti-MSH2 antibody [1B3=3A2B8C] (STJ98252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot detection of MSH2 in K562, CEM, Jurkat, 293T and A431 cell lysates using MSH2 mouse mAb (1:1000 diluted).Predicted band size:100KDa.Observed band size:100KDa.

Anti-MSH2 antibody [4G5-E11-D9] (STJ99224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Anti-MSH2 antibody [ABT21R] (STJ197063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Anti-MSH2 antibody [G219-1129] (STJ16100395)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£889.50 - £1,318.50

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Anti-MSI4/FVE antibody (STJ11106597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Confocal imaging of U-2 OS cells using mSin3A Rabbit monoclonal antibody (STJ11103489, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-mSin3A antibody (1174-1273) [S9MR] (STJ11103489)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using GDF-8/Myostatin Rabbit monoclonal antibody (STJ11104931) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MSTN antibody (24-375) [S4931RM] (STJ11104931)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse lung using GDF-8/Myostatin antibody (STJ28993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MSTN antibody (267-375) (STJ28993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of GDF-8 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-MSTN antibody (38-87 aa) (STJ93255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis using GDF-8 monoclonal antibody against truncated GDF-8-His recombinant protein (1).

Anti-MSTN antibody [6H12] (STJ98105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of CoLo cells using Msx-2 Polyclonal Antibody

Anti-MSX2 antibody (Internal) (STJ94270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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The nature compound dehydrocrenatidine exerts potent antihepatocellular carcinoma by destroying mitochondrial complexes in vitro and in vivo 2022 Feb 02. WB Human

Anti-MT-CO1 antibody (380-460 aa) (STJ190281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit polyclonal antibody (STJ119896) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MT-CO1 antibody (400-500) (STJ119896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using MTCO2 Rabbit polyclonal antibody (STJ113171) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MT-CO2 antibody (101-200) (STJ113171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using MTCO2 antibody (STJ113717) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MT-CO2 antibody (101-200) (STJ113717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of HeLa cells using MTCO2 Rabbit monoclonal antibody (STJ11101958, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-MT-CO2 antibody (128-227) [S8MR] (STJ11101958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options