Anti-ESRRB antibody (434-508) (STJ110714)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ESRRB (434-508) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ESRRB |
| Gene ID | 2103 |
| Uniprot ID | ERR2_HUMAN |
| Immunogen Region | 434-508 |
| Immunogen Sequence | GQEQLRGSPKDERMSSHDGK CPFQSAAFTSRDQSNSPGIP NPRPSSPTPLNERGRQISPS TRTPGGQGKHLWLTM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 434-508 of human ESRRB (NP_004443.3). |
Additional Info
| Post Translational Modifications | Acetylated by PCAF/KAT2 (in vitro). |
| Function | Isoform 3: Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Involved in morula development (2-16 cells embryos) by acting as a regulator at the 8-cell stage. Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells. Also regulates expression of multiple rod-specific genes and is required for survival of this cell type. Plays a role as transcription factor activator of GATA6, NR0B1, POU5F1 and PERM1. Plays a role as transcription factor repressor of NFE2L2 transcriptional activity and ESR1 transcriptional activity. During mitosis remains bound to a subset of interphase target genes, including pluripotency regulators, through the canonical ESRRB recognition (ERRE) sequence, leading to their transcriptional activation in early G1 phase. Can coassemble on structured DNA elements with other transcription factors like SOX2, POU5F1, KDM1A and NCOA3 to trigger ESRRB-dependent gene activation. This mechanism, in the case of SOX2 corecruitment prevents the embryonic stem cells (ESCs) to epiblast stem cells (EpiSC) transition through positive regulation of NR0B1 that inhibits the EpiSC transcriptional program. Also plays a role inner ear development by controlling expression of ion channels and transporters and in early placentation. Isoform 1: Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Positively regulates ESR1 transcriptional activity upon E2 stimulation. |
| Protein Name | Steroid Hormone Receptor Err2Err Beta-2Estrogen Receptor-Like 2Estrogen-Related Receptor BetaErr-BetaNuclear Receptor Subfamily 3 Group B Member 2 |
| Database Links | Reactome: R-HSA-383280 |
| Cellular Localisation | NucleusCytoplasmChromosome |
| Alternative Antibody Names | Anti-Steroid Hormone Receptor Err2 antibodyAnti-Err Beta-2 antibodyAnti-Estrogen Receptor-Like 2 antibodyAnti-Estrogen-Related Receptor Beta antibodyAnti-Err-Beta antibodyAnti-Nuclear Receptor Subfamily 3 Group B Member 2 antibodyAnti-ESRRB antibodyAnti-ERRB2 antibodyAnti-ESRL2 antibodyAnti-NR3B2 antibody |
Information sourced from Uniprot.org