DNA Repair | Research Area | St John's Laboratory

Western blot analysis of lysates from 1) 293, 2) Rat kidney, 3) HELA cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PIAS3 antibody (10-59 aa) (STJ95083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of U2OS cells using PIAS3 antibody (STJ115356).

Anti-PIAS3 antibody (399-628) (STJ115356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of 293F, using PIAS3 antibody (STJ29140) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIAS3 antibody (452-628) (STJ29140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PIAS4 antibody (451-500 aa) (STJ95085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates, using PIAS4 Rabbit polyclonal antibody (STJ27275) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PIAS4 antibody (60-260) (STJ27275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Pirh2 antibody western blot HeLa lysates STJA0037522

Anti-Pirh2 antibody (STJA0037522)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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RCHY1 antibody western blot 293 cells STJA0037462

Anti-Pirh2 antibody [R09-6D1] (STJA0037462)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-PMS-2 antibody IHC human colon adenocarcinoma STJ180591

Anti-PMS-2 antibody [ZR317] (STJ180591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$253.23 - $1,013.61

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Western blot analysis of various lysates using PMS2 Rabbit monoclonal antibody (STJ11102108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PMS2 antibody (1-100) [S8MR] (STJ11102108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of various lysates using PMS2 Rabbit polyclonal antibody (STJ29027) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PMS2 antibody (10-21) (STJ29027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

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Immunohistochemistry of mouse kidney with Anti-PMS2 primary antibody (STJ502561) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-PMS2 antibody (220-270) (STJ502561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-PMS2 antibody (220-270) {Biotin} (STJ502563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-PMS2 antibody (220-270) {FITC} (STJ502564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western blot analysis of extracts of various cell lines, using PMS2 antibody (STJ115635) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PMS2 antibody (390-670) (STJ115635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of various cells using PMS2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PMS2 antibody (461-510 aa) (STJ95169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-PMS2antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 96 kDaObserved band size: 110 kDa

Anti-PMS2 antibody (600-700 aa) [PT2116] (STJ196728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Anti-PMS2 antibody [2G5] (STJA0032685)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

$309.81 - $425.73

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PMS2 antibody immunohistochemistry paraffin-embedded colon tissue STJA0037660

Anti-PMS2 antibody [BP6116] (STJA0037660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$425.73

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Anti-PMS2 antibody [EPR3947] (STJ16101659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$1,936.83

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Anti-PMS2 antibody [IHC422] (STJA0042248)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-p

Reactivity :

Human

$227.01 - $1,008.09

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Anti-PMS2 antibody [M0R4G] (STJ16101787)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,132.29

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Anti-PMS2 antibody [MRQ-28] (STJ16101579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$2,087.25

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Anti-PMS2 antibody [PT0045R] (STJA0039756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$393.30 - $745.20

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PMS2 antibody western blot Jurkat HeLa STJA0033854

Anti-PMS2 antibody [R06-5G3] (STJA0033854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of lysates from wild type (WT) and PMS2 knockout (KO) HeLa cells, using [KO Validated] PMS2 Rabbit monoclonal antibody (STJ11104811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PMS2 antibody [S4811RM] (STJ11104811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunohistochemistry analysis of PMS2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-PMS2 antibody [SM0372] (STJA0010372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$211.83 - $315.33

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Anti-PMS2 antibody [SM5009] (STJA0005009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.33

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Anti-PMS2 antibody [SQab20195] (STJ16102956)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$1,045.35

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Western blot analysis of various lysates using PNKP Rabbit monoclonal antibody (STJ11104200) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PNKP antibody (400-500) [S4200RM] (STJ11104200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-PNKP antibody (50-130 aa) (STJ190167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (STJ28776). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Anti-PNKP antibody (60-320) (STJ28776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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PNKP antibody western blot HeLa A549 HL-60 U251 STJA0035327

Anti-PNKP antibody [R03-0B8] (STJA0035327)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$184.23 - $388.47

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Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (STJ25039). Blue: DAPI for nuclear staining.

Anti-POLB antibody (1-335) (STJ25039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded mouse testis using DNA Polymerase beta Rabbit monoclonal antibody (STJ11101821) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-POLB antibody (236-335) [S1MR] (STJ11101821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various cells using DNA pol Beta Polyclonal Antibody

Anti-POLB antibody (286-335 aa) (STJ92734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using POLD1 Rabbit monoclonal antibody (STJ11102019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-POLD1 antibody (1000-1107) [S9MR] (STJ11102019)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from K562 cells, using POLD1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-POLD1 antibody (1051-1100 aa) (STJ92736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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POLD1 antibody immunocytochemistry K562 cells STJA0033831

Anti-POLD1 antibody [R05-3H1] (STJA0033831)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of extracts of various cell lines, using POLD2 antibody (STJ28916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLD2 antibody (120-360) (STJ28916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-POLD2 antibody (STJA0007746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using POLD3 antibody (STJ29386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-POLD3 antibody (197-466) (STJ29386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of hela cells using DNA pol Delta 3 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLD3 antibody (411-460 aa) (STJ92737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from MCF-7 cells, using POLD4 Rabbit pAb (STJ110804) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-POLD4 antibody (1-107) (STJ110804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLD4 antibody (1-50 aa) (STJ190750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-POLD4 antibody (43-93 aa) (STJ196496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLE1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-POLE antibody (1261-1310 aa) (STJ92739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using POLE2 Rabbit polyclonal antibody (STJ114708) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLE2 antibody (1-290) (STJ114708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

Choose Options

Western blot analysis of extracts from Jurkat cells, using DNA pol Epsilon B Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLE2 antibody (Internal) (STJ96430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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