• Western blot analysis of various lysates using POLE2 Rabbit polyclonal antibody (STJ114708) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLE2 antibody (1-290) (STJ114708)

SKU:
STJ114708

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLE2 (1-290) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLE2
Gene ID: 5427
Uniprot ID: DPOE2_HUMAN
Immunogen Region: 1-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2).
Immunogen Sequence: MAPERLRSRALSAFKLRGLL LRGEAIKYLTEALQSISELE LEDKLEKIINAVEKQPLSSN MIERSVVEAAVQECSQSVDE TIEHVFNIIGAFDIPRFVYN SERKKFLPLLMTNHPAPNLF GTPRDKAEMFRERYTILHQR THRHELFTPPVIGSHPDESG SKFQLKTIETLLGSTTKIGD AIVLGMITQLKEGKFFLEDP TGTVQLDLSKAQFHSGLYTE ACFVLAEGWFEDQVFHVNA
Function Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication.
Protein Name Dna Polymerase Epsilon Subunit 2
Dna Polymerase Ii Subunit 2
Dna Polymerase Epsilon Subunit B
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-5651801
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-68952
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Polymerase Epsilon Subunit 2 antibody
Anti-Dna Polymerase Ii Subunit 2 antibody
Anti-Dna Polymerase Epsilon Subunit B antibody
Anti-POLE2 antibody
Anti-DPE2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance