Disease | Research Area | St John's Laboratory

Western blot analysis of extracts of 293T cells, using LC3B antibody (STJ113056) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MAP1LC3B antibody (1-125) (STJ113056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of 293T and 293T Chloroquine cells using LC3B Rabbit polyclonal antibody (STJ113754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP1LC3B antibody (1-125) (STJ113754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using LC3B Mouse monoclonal antibody (STJ119545) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MAP1LC3B antibody (1-125) [S5MM] (STJ119545)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of lysates from C6 cells using MAP1LC3B Rabbit polyclonal antibody (STJ11104549) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAP1LC3B antibody (1-50) (STJ11104549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

Choose Options

Anti-MAP1LC3B antibody (2-107) [S4MR] (STJ11101754)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Anti-MAP1LC3B antibody (STJ13101341)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Jia, Wei‐Qiang, et al. "Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade. " Journal of cellular and molecular medicine 24. 5 (2020) : 2901-2916.

Anti-MAP1LC3B antibody (STJ197355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using LC3B Mouse mAb diluted at 1:200.

Anti-MAP1LC3B antibody [9H5] (STJ97748)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Cow

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of MEK1 in paraffin-embedded mouse kidney using [KO Validated] MEK1 Rabbit polyclonal antibody (STJ114560) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP2K1 antibody (1-100) (STJ114560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of U2OS cells using MEK1 Rabbit polyclonal antibody (STJ24463). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K1 antibody (1-100) (STJ24463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g MEK1 antibody (STJ11101700). Western blot was performed from the immunoprecipitate using MEK1 (STJ11101700) at a dilution of 1:1000.

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of MEK1 in (1) A431 lysates; (2) HeLa lysates using MEK1 antibody.

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog

£264.50

Choose Options

Western blot analysis of 293T-UV cells using MEK-1 Polyclonal Antibody diluted at 1：500

Anti-MAP2K1 antibody (252-301 aa) (STJ94076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry of mouse brain with Anti-MAP2K1 primary antibody (STJ501657) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MAP2K1 antibody (300-350) (STJ501657)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-MAP2K1 antibody (300-350) {Biotin} (STJ501658)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-MAP2K1 antibody (300-350) {FITC} (STJ501659)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K2 antibody (1-100) (STJ116970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of mouse brain, using MEK2 antibody (STJ24464). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MAP2K2 antibody (1-100) (STJ24464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP2K2 antibody (1-100) [S5MR] (STJ11101615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

Choose Options

Western blot analysis of mouse-lung KB 293T lysis using MEK-2 antibody. Antibody was diluted at 1:2000

Anti-MAP2K2 antibody (261-310 aa) (STJ94081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue with MEK2 Mouse mAb diluted at 1:2, 000.

Anti-MAP2K2 antibody [2C3] (STJ97518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis using MEK-2 monoclonal antibody against PC-12 (1) , Jurkat (2) , HeLa (3) and NIH/3T3 (4) cell lysate.

Anti-MAP2K2 antibody [7F5] (STJ98237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

Choose Options

Western blot analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MAP2K4 antibody (STJ116981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MAP2K4 antibody (300-399) (STJ116981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g MAP2K4 antibody (STJ110035). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (STJ110035) at a dilution of 1:1000.

Anti-MAP2K4 antibody (301-399) (STJ110035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using MEK-4 Polyclonal Antibody diluted at 1：1000

Anti-MAP2K4 antibody (46-95 aa) (STJ94084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human thymoma tissue (left) and spleen tissue (right) , showing cytoplasmic localization with DAB staining using MEK-4 monoclonal antibody.

Anti-MAP2K4 antibody [2D10D8/4G11B2/2D10C4/2D10C3] (STJ98238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis using MEK-4 monoclonal antibody against HepG2 (1) , K562 (2) , and HEK293 (3) cell lysate.

Anti-MAP2K4 antibody [5H4] (STJ98239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Immunofluorescence analysis of L929 cells using MEK7 antibody (STJ24470) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K7 antibody (1-419) (STJ24470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of 293 cells using MEK-7 Polyclonal Antibody

Anti-MAP2K7 antibody (241-290 aa) (STJ94090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from wild type (WT) and MEK7 knockout (KO) 293T (KO) cells, using [KO Validated] MEK7 Rabbit monoclonal antibody (STJ11101420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAP2K7 antibody (320-419) [S0MR] (STJ11101420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of lysates from Mouse thymus, using MAP3K11 Rabbit polyclonal antibody (STJ118681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP3K11 antibody (140-260) (STJ118681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MLK3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K11 antibody (640-689) (STJ94151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of U2OS cells using TAK1 Rabbit polyclonal antibody (STJ113923) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP3K7 antibody (110-325) (STJ113923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, Tak1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MAP3K7 antibody (161-210 aa) (STJ95901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using TAK1 antibody (STJ117999). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MAP3K7 antibody (510-606) (STJ117999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of TAK1 in A431 using MAP3K7 antibody.

Anti-MAP3K7 antibody (550-606 aa) [R09-4G4] (STJA0014935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£264.50

Choose Options

Western blot analysis of TAK1 in Hela, A431 lysates using TAK1 antibody.

Anti-MAP3K7 antibody (593-606 aa) [R09-4B6] (STJA0012028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£264.50

Choose Options

Western blot analysis of 3T3 cells using ERK 2 Polyclonal Antibody diluted at 1：2000

Anti-MAPK1 antibody (136-185 aa) (STJ92992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK1 antibody (200-300) (STJ113009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using ERK2 antibody (STJ11101738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK1 antibody (200-300) [S8MR] (STJ11101738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot with Anti-MAPK1 Antibody (STJ501678) of mouse brain at 1:500 dilution in buffer. Second band may represent phosphorylated MAPK1.

Anti-MAPK1 antibody (300-350) (STJ501678)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-MAPK1 antibody (300-350) {Biotin} (STJ501679)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-MAPK1 antibody (300-350) {FITC} (STJ501680)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-MAPK1 antibody (C-Term) [M233] (STJA0005410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£390.50

Choose Options

Western blot analysis using ERK 2 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , MCF-7 (3) , HEK293 (4) , Jurkat (5) , A549 (6) , NTERA-2 (7) and SMMC-7721 (8) cell lysate.

Anti-MAPK1 antibody [4C11] (STJ98047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

Choose Options

Western blot analysis of extracts of various cell lines, using p38 MAPK rabbit monoclonal antibody (STJ11102152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MAPK14 antibody (100-200) [S2MR] (STJ11102152)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, p38 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-MAPK14 antibody (147-196 aa) (STJ94878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish/Pig

£48.00 - £252.00

Choose Options