Anti-GBF1 antibody (STJA0007688)

SKU:
STJA0007688

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GBF1
Gene ID: 8729
Uniprot ID: GBF1_HUMAN
Specificity: This antibody detects endogenous levels of GBF1 at Human
Immunogen: Synthesized peptide derived from human GBF1
Post Translational Modifications AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis.
Function Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway.its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport.the function requires its GEF activity (probably at least in part on ARF4 and ARF5). Has GEF activity towards ARF1. Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP. Required for the assembly of the Golgi apparatus. The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions. May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets.however, this function is under debate. In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex. Plays a role in maintaining mitochondrial morphology.
Protein Name Golgi-Specific Brefeldin A-Resistance Guanine Nucleotide Exchange Factor 1
Bfa-Resistant Gef 1
Database Links Reactome: R-HSA-199992
Reactome: R-HSA-5620916
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Cis-Golgi Network
Endoplasmic Reticulum-Golgi Intermediate Compartment
Trans-Golgi Network
Cytoplasm
Lipid Droplet
Membrane
Peripheral Membrane Protein
Cycles Rapidly On And Off Early Golgi Membranes
Stabilized On Membranes When Complexed With Arf1-Gdp And Is Released From Both Arf1 And Membranes After It Catalyzes Gdp Displacement And Arf1 Binds Gtp
Continuous Cycles Of Recruitment And Dissociation Of Gbf1 To Membranes Are Required For Sustained Arf Activation And Cop I Recruitment
In Neutrophils Is Translocated From The Golgi To The Leading Edge Upon Gpcr Stimulation
Localization To Lipid Droplets Is Questionable
Alternative Antibody Names Anti-Golgi-Specific Brefeldin A-Resistance Guanine Nucleotide Exchange Factor 1 antibody
Anti-Bfa-Resistant Gef 1 antibody
Anti-GBF1 antibody
Anti-KIAA0248 antibody

Information sourced from Uniprot.org

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