• Western blot analysis of various lysates using Glucokinase (GCK) Rabbit polyclonal antibody (STJ117253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GCK antibody (250-465) (STJ117253)

SKU:
STJ117253

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCK (250-465) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCK
Gene ID: 2645
Uniprot ID: HXK4_HUMAN
Immunogen Region: 250-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Glucokinase (Glucokinase (GCK) ) (NP_000153.1).
Immunogen Sequence: RMCVNTEWGAFGDSGELDEF LLEYDRLVDESSANPGQQLY EKLIGGKYMGELVRLVLLRL VDENLLFHGEASEQLRTRGA FETRFVSQVESDTGDRKQIY NILSTLGLRPSTTDCDIVRR ACESVSTRAAHMCSAGLAGV INRMRESRSEDVMRITVGVD GSVYKLHPSFKERFHASVRR LTPSCEITFIESEEGSGRGA ALVSAVACKKACMLGQ
Function Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.
Protein Name Hexokinase-4
Hk4
Glucokinase
Hexokinase Type Iv
Hk Iv
Hexokinase-D
Database Links Reactome: R-HSA-170822
Reactome: R-HSA-210745
Reactome: R-HSA-5619073
Reactome: R-HSA-5619107
Reactome: R-HSA-70171
Reactome: R-HSA-9615017
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Under Low Glucose Concentrations
Gck Associates With Gckr And The Inactive Complex Is Recruited To The Hepatocyte Nucleus
Alternative Antibody Names Anti-Hexokinase-4 antibody
Anti-Hk4 antibody
Anti-Glucokinase antibody
Anti-Hexokinase Type Iv antibody
Anti-Hk Iv antibody
Anti-Hexokinase-D antibody
Anti-GCK antibody

Information sourced from Uniprot.org

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