Developmental Biology | Research Area | St John's Laboratory

Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-KIT-Tyr823 antibody (STJ196276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KIT-Tyr936 antibody (906-955 aa) (STJ90817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (STJ22223). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-KIT-Y721 antibody (STJ22223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-KRT17-Ser44 antibody (STJ196342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HT-29 cells, using Phospho-Cytokeratin 18 (KRT18) -S33 Rabbit pAb (STJ11105775). HT-29 cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-KRT18-S33 antibody (STJ11105775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-Cytokeratin 18 (S33) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-KRT18-Ser33 antibody (1-50 aa) (STJ91019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 293T using p-Cytokeratin 18 (S52) antibody. Antibody was diluted at 1:500

Anti-Phospho-KRT18-Ser52 antibody (21-70 aa) (STJ90707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-KRT20-Ser13 antibody (STJ196343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Hep G2, using Phospho-Cytokeratin 8 (KRT8) -S74 Rabbit polyclonal antibody (STJ11105809) at 1:1000 dilution. Hep G2 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-KRT8-S74 antibody (STJ11105809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-Cytokeratin 8 (S432) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-KRT8-Ser432 antibody (401-450 aa) (STJ91078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1：500

Anti-Phospho-KRT8-Ser73 antibody (41-90 aa) (STJ90242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, using CD171/N-CAML1 (Phospho-Ser1181) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-L1CAM-Ser1181 antibody (1147-1196 aa) (STJ90507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-LEF-1 (S42) Polyclonal Antibody

Anti-Phospho-LEF1-Ser42 antibody (8-57 aa) (STJ90534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-LIMK1-Ser-323 antibody (STJA0005461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western Blot analysis of 293T using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-LIMK1-Ser310 antibody (STJA0009991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK1-T508 Rabbit pAb (STJ110833).

Anti-Phospho-LIMK1-T508 antibody (STJ110833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-LIMK1-Thr-508 antibody (STJA0005462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of various cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LIMK1-Thr508 antibody (471-520 aa) (STJ90321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of LIMK2 (Phospho-Ser283) Antibody. The lane on the right is blocked with the LIMK2 (Phospho-Ser283) peptide.

Anti-Phospho-LIMK2-Ser283 antibody (249-298 aa) (STJ91188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK2-T505 Rabbit pAb (STJ22229). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-LIMK2-T505 antibody (STJ22229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-LIMK-2 (T505) Polyclonal Antibody

Anti-Phospho-LIMK2-Thr505 antibody (STJ90322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-LIN28A-Ser200 antibody (STJ194717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-Lyn antibody (425-525) (STJ502546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Lyn antibody (425-525) {Biotin} (STJ502547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Lyn antibody (425-525) {FITC} (STJ502548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-LYN-Tyr507 antibody (STJ196350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Lyn (Y508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LYN-Tyr508 antibody (463-512 aa) (STJ90764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (STJ11102620) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-LYN-Y396 antibody [S0MR] (STJ11102620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Lyn-Y507 antibody (STJ11107669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (STJ22232) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-S217 Rabbit pAb (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (STJ22231) at 1:1000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (STJ22233) at 1:2000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. PC-12 cells were treated by Nocodazole (1 Mu g/mL) at 37 °C for 16 hours or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

£454.50

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

£454.50

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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

£454.50

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Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (STJ22237). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K2-T394 antibody (STJ22237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K2-Thr394 antibody (261-310 aa) (STJ90329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HL60 cells, using Phospho-MEK6-S207 Rabbit pAb (STJ22247). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K6-S207 antibody (STJ22247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MAP2K6-Ser207 antibody (STJ90333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western Blot analysis of 1, HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPK12-Tyr185/182 antibody (STJA0009932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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