Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-Src-S17 Rabbit pAb (STJ113487) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SRC-S17 antibody (STJ113487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-SRC-Ser17 antibody (STJ196435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of COLO cells using Phospho-c-Src (S75) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Ser75 antibody (41-90 aa) (STJ90958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-SRC-Tyr-215 antibody (STJA0005602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-SRC-Tyr-530 antibody (C-Term) [M261] (STJA0005603)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using Src (Phospho-Tyr216) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SRC-Tyr216 antibody (181-230 aa) (STJ90611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Jurkat cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SRC-Tyr419 antibody (386-435 aa) (STJ90237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Tyr419 antibody (STJ90446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-SRC-Tyr529 antibody (487-536 aa) (STJ90238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (STJ11102608) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SRC-Y419 antibody [S8MR] (STJ11102608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (STJ22407) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SRC-Y529 antibody (STJ22407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of A-431, using Phospho-Src-Y529 antibody (STJ11105854) at 1:10000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SRC-Y529 antibody [S5854RM] (STJ11105854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western Blot analysis of HUVEC cell, HEK-293T cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SREBF2-Ser455 antibody (STJA0009941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-SRF-S103 Rabbit pAb (STJ117927) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-SRF-S103 antibody (STJ117927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SRF-Ser103 antibody (71-120 aa) (STJ90771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using SRF (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SRF-Ser77 antibody (41-90 aa) (STJ90935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SRF-Thr159 antibody (126-175 aa) (STJ91269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of 1 HepG2 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SRPK1-Thr601 antibody (STJA0009934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot with Anti-Phospho-STAT3 Antibody (STJ502696) of THP cells. Antibody at 1:500 dilution in buffer.

Anti-Phospho-STAT3 antibody (700-750) (STJ502696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-STAT3 antibody (700-750) {Biotin} (STJ502697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-STAT3 antibody (700-750) {FITC} (STJ502698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (STJ116371) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-STAT3-S727 antibody (STJ116371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (STJ11102565) at 1:1000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-STAT3-S727 antibody [S5MR] (STJ11102565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, Stat3 (phospho Ser727) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STAT3-Ser727 antibody (694-743 aa) (STJ90410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-STAT3-Ser727 antibody [PT0911R] (STJA0039802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-STAT3-Ser754 antibody (STJ196440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of mouse-spleen tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-STAT3-Tyr705 antibody (672-721 aa) (STJ90411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Anti-Phospho-STAT3-Tyr705 antibody [PT0742R] (STJA0039211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Muscle Actin Mouse mAb diluted at 1:200.

Anti-Phospho-STAT3-Y705 antibody (640-720 aa) [1E9] (STJ97747)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-Y705 Rabbit pAb (STJ27511) at 1:500 dilution. HeLa cells were treated by IFN-Alpha (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT3-Y705 antibody (STJ27511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (STJ11102563) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT3-Y705 antibody [S3MR] (STJ11102563)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-STAT3-Y705 Rabbit monoclonal antibody (STJ11105819) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-STAT3-Y705 antibody [S5819RM] (STJ11105819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-STAT3-Y705 antibody [SAA0302] (STJA0019171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£378.50 - £1,632.50

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-STAT4-Ser721 antibody (STJA0010068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Stat4 (Y693) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-STAT4-Tyr693 antibody (660-709 aa) (STJ90412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STAT4-Tyr693 antibody (STJ90753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STX1A-Ser14 antibody (1-50 aa) (STJ90729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using TAL-1 (Phospho-Ser122) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TAL1-Ser122 antibody (96-145 aa) (STJ90638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using E2A (Phospho-Thr355) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TCF3-Thr355 antibody (321-370 aa) (STJ91252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Telomerase (Phospho-Ser227) Antibody. The lane on the right is blocked with the Telomerase (Phospho-Ser227) peptide.

Anti-Phospho-TERT-Ser227 antibody (196-245 aa) (STJ91354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from COLO205 cells, using Telomerase (Phospho-Ser824) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TERT-Ser824 antibody (796-845 aa) (STJ90548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-CD71 (S24) Polyclonal Antibody

Anti-Phospho-TFRC-Ser24 antibody (15-64 aa) (STJ90889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-TLN1-Ser-425 antibody (STJA0005613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-TLN1-Ser425 antibody (STJ196447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of 293T cells, using Phospho-Tp53-S15 antibody (STJ11101082) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-TP53-S15 antibody (STJ11101082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells using Phospho-p53-S15 Rabbit pAb (STJ22433) at 1:900 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-TP53-S15 antibody (STJ22433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-p53-S315 Rabbit pAb (STJ22439). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-TP53-S315 antibody (STJ22439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit pAb (STJ25927). HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S33 antibody (STJ116382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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