Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using p90Rsk/RSK1/RPS6KA1 Rabbit monoclonal antibody (STJ11102133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RPS6KA1 antibody (1-100) [S3MR] (STJ11102133)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-RPS6KA1 antibody (539-588 aa) (STJ95545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using p90Rsk/RSK1/RPS6KA1 Rabbit polyclonal antibody (STJ118164) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RPS6KA1 antibody (C-Term) (STJ118164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Rsk-1 monoclonal antibody against truncated Rsk-1 recombinant protein.

Anti-RPS6KA1 antibody [10B1D7A9/10B1D7E12/10B1D7] (STJ98366)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of p90RSK in COS7, 3T3, Jurkat, Hela and A549 cell lysates using p90RSK mouse mAb (dilution 1:1000).Predicted band size:90KDa.Observed band size:90KDa.

Anti-RPS6KA1 antibody [6B9-D6-F8] (STJ99265)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis of hepg2, NT28 cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-RPS6KA2 antibody (330-400 aa) (STJ98670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using RPS6KA2 Rabbit polyclonal antibody (STJ118735) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RPS6KA2 antibody (632-733) (STJ118735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody (STJ27389). Blue: DAPI for nuclear staining.

Anti-RPS6KA3 antibody (68-327) (STJ27389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-RPS6KA3 antibody (STJA0010282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Rsk-2 monoclonal antibody against HeLa (1) , MCF-7 (2) , and HepG2 (3) cell lysate.

Anti-RPS6KA3 antibody [4E10] (STJ98367)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from Raw264.7 cells, treated with UV 5', using MSK2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-RPS6KA4 antibody (531-580) (STJ94268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse lung using RPS6KA4 antibody (STJ112169) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPS6KA4 antibody (673-772) (STJ112169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using MSK1 Polyclonal Antibody diluted at 1：1000

Anti-RPS6KA5 antibody (551-600) (STJ94267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using RPS6KA5 Rabbit monoclonal antibody (STJ11105249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPS6KA5 antibody (639-736) [S5249RM] (STJ11105249)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£129.50 - £574.50

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Western blot analysis of C6, using RPS6KA5 antibody (STJ27666) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS6KA5 antibody (700-800) (STJ27666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from mouse kidney, using RPS6KA6 Rabbit polyclonal antibody (STJ117076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-RPS6KA6 antibody (646-745) (STJ117076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Rsk-4 Polyclonal Antibody diluted at 1：500

Anti-RPS6KA6 antibody (661-710 aa) (STJ95550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using RPS7 antibody (STJ28814). Blue: DAPI for nuclear staining.

Anti-RPS7 antibody (1-194) (STJ28814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RPS7 antibody (50-130 aa) (STJ190474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using RPS8 Rabbit polyclonal antibody (STJ11100330) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RPS8 antibody (10-100) (STJ11100330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Ribosomal Protein S8 Polyclonal Antibody diluted at 1：2000

Anti-RPS8 antibody (111-160 aa) (STJ95506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using RPS9 antibody (STJ11104268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RPS9 antibody (1-162) (STJ11104268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Ribosomal Protein S9 Polyclonal Antibody diluted at 1：500

Anti-RPS9 antibody (31-80 aa) (STJ95507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (STJ113608) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RPSA antibody (1-295) (STJ113608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Laminin-R antibody in paraffin-embedded human tonsil tissue.

Anti-RPSA antibody (166-215 aa) (STJ93898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using Repetin Polyclonal Antibody

Anti-RPTN antibody (648-697 aa) (STJ95417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-RRAS antibody (1-80 aa) (STJ190169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot with Anti-RUNX1 Antibody (STJ502834) with RUNX1 peptide. Antibody at 1:500 dilution in buffer.

Anti-RUNX1 antibody (1-80) (STJ502834)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey

£427.50

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Anti-RUNX1 antibody (1-80) {Biotin} (STJ502835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey

£528.50

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Anti-RUNX1 antibody (1-80) {FITC} (STJ502836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey

£528.50

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Western blot analysis of various lysates using RUNX1 Rabbit mAb (STJ11107270) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 3s.

Anti-RUNX1 antibody (101-200 aa) [SRM] (STJ11107270)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates using RUNX1 Rabbit polyclonal antibody (STJ113542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RUNX1 antibody (150-250) (STJ113542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using RUNX1 antibody (STJ25422). Blue: DAPI for nuclear staining.

Anti-RUNX1 antibody (171-260) (STJ25422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Runx1 antibody (200-260 aa) (STJ13100304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunofluorescence analysis of HeLa cells, using AML1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-RUNX1 antibody (269-318 aa) (STJ95555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Runx1 antibody (Internal Region) (STJ13101911)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of various lysates using RUNX1 Rabbit pAb (STJ11107007) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Negative control (NC) : SH-SY5Y. <br>Exposure time: 60s.

Anti-RUNX1 antibody (STJ11107007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-Runx1 antibody (STJ13100301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Runx1 antibody (STJ13101912)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis using RUNX1 monoclonal antibody against Jurkat cell lysate.

Anti-RUNX1 antibody [5A1] (STJ98371)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using RXR Alpha antibody (STJ117436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-RXRA antibody (1-100) (STJ117436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR Alpha antibody (STJ11101642) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RXRA antibody (1-100) [S2MR] (STJ11101642)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-RXRA antibody (200-280 aa) (STJ190122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Duck

£48.00 - £252.00

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Anti-SALL1 antibody (STJ192081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human seminoma stained with anti-SALL4 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Anti-SALL4 antibody (1-185aa) [ZR276] (STJ180538)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£162.50 - £583.50

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Western blot analysis of various lysates using Sall4 Rabbit pAb (STJ29204) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-SALL4 antibody (1-220) (STJ29204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-SALL4 antibody (400-450) (STJ507826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-SALL4 antibody (400-450) {Biotin} (STJ507860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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