Anti-Phospho-CDH2-Tyr-820 antibody (STJA0005287)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from t
General Information
| Short Description | Rabbit polyclonal antibody anti-Phospho-N-Cadherin-Tyr-820 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Applications | WB/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDH2 |
| Gene ID | 1000 |
| Uniprot ID | CADH2_HUMAN |
| Immunogen | Phospho-N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from t |
| Specificity | This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-860) and unphosphorylated N-cadherin (Tyr-820) peptides before affinity purification using phospho-N-cadherin (Tyr-820) peptide. The purified antibody detects a 130 kDa* band corresponding t |
Additional Info
| Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. |
| Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Protein Name | Cadherin-2Cdw325Neural CadherinN-CadherinCd Antigen Cd325 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-418990Reactome: R-HSA-525793Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSarcolemmaCell JunctionCell SurfaceDesmosomeAdherens JunctionColocalizes With Tmem65 At The Intercalated Disk In CardiomyocytesColocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes |
| Alternative Antibody Names | Anti-Cadherin-2 antibodyAnti-Cdw325 antibodyAnti-Neural Cadherin antibodyAnti-N-Cadherin antibodyAnti-Cd Antigen Cd325 antibodyAnti-CDH2 antibodyAnti-CDHN antibodyAnti-NCAD antibody |
Information sourced from Uniprot.org