Anti-Phospho-CD44-Ser706 antibody (681-730 aa) (STJ90509)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CD44 around the phosphorylation site of Ser706 at the amino acid range 681-730
General Information
| Short Description | Rabbit polyclonal anti-Phospho-CD44 antigen-Ser706 (681-730 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD44 |
| Gene ID | 960 |
| Uniprot ID | CD44_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CD44 around the phosphorylation site of Ser706 at the amino acid range 681-730 |
| Immunogen Region | 681-730 aa |
| Specificity | Phospho-CD44 (S706) Polyclonal Antibody detects endogenous levels of CD44 protein only when phosphorylated at S706. |
Additional Info
| Post Translational Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
| Function | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Protein Name | Cd44 AntigenCdw44EpicanExtracellular Matrix Receptor IiiEcmr-IiiGp90 Lymphocyte Homing/Adhesion ReceptorHutch-IHeparan Sulfate ProteoglycanHermes AntigenHyaluronate ReceptorPhagocytic Glycoprotein 1Pgp-1Phagocytic Glycoprotein IPgp-ICd Antigen Cd44 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2160916Reactome: R-HSA-6798695Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusSecretedColocalizes With Actin In Membrane Protrusions At Wounding EdgesCo-Localizes With RdxEzr And Msn In MicrovilliLocalizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains |
| Alternative Antibody Names | Anti-Cd44 Antigen antibodyAnti-Cdw44 antibodyAnti-Epican antibodyAnti-Extracellular Matrix Receptor Iii antibodyAnti-Ecmr-Iii antibodyAnti-Gp90 Lymphocyte Homing/Adhesion Receptor antibodyAnti-Hutch-I antibodyAnti-Heparan Sulfate Proteoglycan antibodyAnti-Hermes Antigen antibodyAnti-Hyaluronate Receptor antibodyAnti-Phagocytic Glycoprotein 1 antibodyAnti-Pgp-1 antibodyAnti-Phagocytic Glycoprotein I antibodyAnti-Pgp-I antibodyAnti-Cd Antigen Cd44 antibodyAnti-CD44 antibodyAnti-LHR antibodyAnti-MDU2 antibodyAnti-MDU3 antibodyAnti-MIC4 antibody |
Information sourced from Uniprot.org