Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (STJ22357) at 1:1000 dilution. Jurkat cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2B-Y402 antibody (STJ22357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-Pyk2-Y402 Rabbit pAb (STJ29378) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PTK2B-Y402 antibody (STJ29378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-Brk (Y447) Polyclonal Antibody

Anti-Phospho-PTK6-Tyr447 antibody (402-451 aa) (STJ90697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot of mouse testicular lysate showing specific immunolabeling of the ~104 kDa PTPH1 phosphorylated at Ser459 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 400 units/100ul lysate for 30 minutes, RT).

Anti-Phospho-PTPH1-Ser459 antibody (STJA0003758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Sheep

£454.50

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Anti-Phospho-PTPN1-Ser-50 antibody (STJA0005553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of COLO cells using Phospho-PTP1B (S50) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PTPN1-Ser50 antibody (16-65 aa) (STJ90882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-PTPN1-Tyr-152 antibody (STJA0005554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y542) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTPN11-Tyr542 antibody (508-557 aa) (STJ90741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y580) Polyclonal Antibody

Anti-Phospho-PTPN11-Tyr580 antibody (546-595 aa) (STJ90742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SHP2-Y542 Rabbit pAb (STJ22358) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PTPN11-Y542 antibody (STJ22358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-PTPN6-Ser-591 antibody (STJA0005582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PTPN6-Tyr-536 antibody (STJA0005581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of RAW264.7+EGF cells using Phospho-SH-PTP1 (Y536) Polyclonal Antibody

Anti-Phospho-PTPN6-Tyr536 antibody (502-551 aa) (STJ90740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SHP-1 (Phospho-Tyr564) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTPN6-Tyr564 antibody (530-579 aa) (STJ90575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey

£48.00 - £252.00

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Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-SHP1-Y536 antibody (STJ22360).

Anti-Phospho-PTPN6-Y536 antibody (STJ22360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa and RAW 264. 7 cells, using Phospho-SHP1-Y564 Rabbit pAb (STJ117885) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour. RAW 264. 7 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PTPN6-Y564 antibody (STJ117885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells treated with serum 20% 15', using PTPRA (Phospho-Tyr798) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTPRA-Tyr798 antibody (753-802 aa) (STJ90477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 Rabbit pAb (STJ117912) at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PTPRA-Y789 antibody (STJ117912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of HeLa cells, using CD45 (Phospho-Ser1007) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTPRC-Ser1007 antibody (981-1030 aa) (STJ90703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PXN-Ser-178 antibody (STJA0005517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PXN-Ser-83 antibody (STJA0005515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Mouse

£367.50

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Anti-Phospho-PXN-Ser178 antibody (STJ90613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PXN-Ser272 antibody (STJ90604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PXN-Thr-538 antibody (STJA0005518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-PXN-Tyr 118 antibody (STJA0005516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-PXN-Tyr-31 antibody [M102] (STJA0005514)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various cells using Phospho-Paxillin (Y118) Polyclonal Antibody

Anti-Phospho-PXN-Tyr118 antibody (85-134 aa) (STJ90379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of various cells using Phospho-Paxillin (Y31) Polyclonal Antibody diluted at 1:1000

Anti-Phospho-PXN-Tyr31 antibody (15-64 aa) (STJ90380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Paxillin (Y88) Polyclonal Antibody

Anti-Phospho-PXN-Tyr88 antibody (STJ90381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-Paxillin-Y118 Rabbit pAb (STJ22361) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PXN-Y118 antibody (STJ22361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Paxillin-Y118 Rabbit mAb (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PXN-Y118 antibody [S8MR] (STJ11102648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Pycard-Tyr-144 antibody (STJA0005271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (STJ22364) at 1:1000 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RAC1-S71 antibody (STJ22364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of Rac1 (Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody (STJ99666 was diluted at 1:10000, 37° 1hour.

Anti-Phospho-RAC1-Ser71 antibody (STJA0009870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RACGAP1-Ser387 antibody (353-402 aa) (STJ91093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD17-Ser635 antibody (STJ196393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Rad17 (S645) Polyclonal Antibody

Anti-Phospho-RAD17-Ser645 antibody (621-670 aa) (STJ90451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from K562 cells, using Phospho-Rad17 (S646) Polyclonal Antibody.

Anti-Phospho-RAD17-Ser646 antibody (STJ91363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD18-Ser403 antibody (STJ196394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD50-Ser635 antibody (STJ196395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HELA-UV 293T-UV using p-Rad51 (T309) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RAD51-Thr309 antibody (275-324 aa) (STJ91266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using RAD51 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAD51-Tyr315 antibody (281-330 aa) (STJ90536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using RAD52 (Phospho-Tyr104) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAD52-Tyr104 antibody (70-119 aa) (STJ90918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Anti-Phospho-RAD9-Ser272 antibody (STJA0009820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (STJ113482) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S259 antibody (STJ113482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (STJ11102598) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RAF1-S259 antibody [S8MR] (STJ11102598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Anti-Phospho-RAF1-S296 antibody (STJ11101085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Raf1-S338 pAb (STJ117871) at 1:1000 dilution or Phospho-Raf1-S338 antibody (STJ25284). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-RAF1-S338 antibody (STJ117871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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