• Western blot analysis of lysates from LOVO cells treated with PMA 125ng/ml 30', using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK5/6-Ser602/S560 antibody (566-615 aa) (STJ90635)

SKU:
STJ90635

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase PAK 5 and Serine/threonine-protein kinase PAK 6-Ser602/S560 (566-615 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAK5
PAK6
Gene ID: 57144
106821730/56924
Uniprot ID: PAK5_HUMAN
PAK6_HUMAN
Immunogen Region: 566-615 aa
Specificity: Phospho-PAK5/6 (S602/S560) Polyclonal Antibody detects endogenous levels of PAK5/6 protein only when phosphorylated at S602/S560.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PAK5/6 around the phosphorylation site of Ser602/Ser560 at the amino acid range 566-615
Post Translational Modifications Autophosphorylated when activated by CDC42/p21.
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
Protein Name Serine/Threonine-Protein Kinase Pak 5
P21-Activated Kinase 5
Pak-5
P21-Activated Kinase 7
Pak-7
Database Links Reactome: R-HSA-428540
Cellular Localisation Mitochondrion
Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Mitochondria
And Mitochondrial Localization Is Essential For The Role In Cell Survival
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 5 antibody
Anti-P21-Activated Kinase 5 antibody
Anti-Pak-5 antibody
Anti-P21-Activated Kinase 7 antibody
Anti-Pak-7 antibody
Anti-PAK5 antibody
Anti-KIAA1264 antibody
Anti-PAK7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance