• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody (STJ117913) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y1021 Rabbit polyclonal antibody (STJ117913) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PDGFRB-Y1021 antibody (STJ117913)

SKU:
STJ117913

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PDGFRB-Y1021 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDGFRB
Gene ID: 5159
Uniprot ID: PGFRB_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y1021 of human Phospho-PDGFRB-Y1021 (NP_002600.1).
Immunogen Sequence: NDYII
Post Translational Modifications Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-579, and to a lesser degree, at Tyr-581, is important for interaction with SRC family kinases. Phosphorylation at Tyr-740 and Tyr-751 is important for interaction with PIK3R1. Phosphorylation at Tyr-751 is important for interaction with NCK1. Phosphorylation at Tyr-771 and Tyr-857 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-857 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1009 is important for interaction with PTPN11. Phosphorylation at Tyr-1009 and Tyr-1021 is important for interaction with PLCG1. Phosphorylation at Tyr-1021 is important for interaction with CBL.PLCG1 and CBL compete for the same binding site. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. Dephosphorylated by PTPN2 at Tyr-579 and Tyr-1021. N-glycosylated. Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation.
Function Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Protein Name Platelet-Derived Growth Factor Receptor Beta
Pdgf-R-Beta
Pdgfr-Beta
Beta Platelet-Derived Growth Factor Receptor
Beta-Type Platelet-Derived Growth Factor Receptor
Cd140 Antigen-Like Family Member B
Platelet-Derived Growth Factor Receptor 1
Pdgfr-1
Cd Antigen Cd140b
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-186763
Reactome: R-HSA-186797
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Lysosome Lumen
After Ligand Binding
The Autophosphorylated Receptor Is Ubiquitinated And Internalized
Leading To Its Degradation
Alternative Antibody Names Anti-Platelet-Derived Growth Factor Receptor Beta antibody
Anti-Pdgf-R-Beta antibody
Anti-Pdgfr-Beta antibody
Anti-Beta Platelet-Derived Growth Factor Receptor antibody
Anti-Beta-Type Platelet-Derived Growth Factor Receptor antibody
Anti-Cd140 Antigen-Like Family Member B antibody
Anti-Platelet-Derived Growth Factor Receptor 1 antibody
Anti-Pdgfr-1 antibody
Anti-Cd Antigen Cd140b antibody
Anti-PDGFRB antibody
Anti-PDGFR antibody
Anti-PDGFR1 antibody

Information sourced from Uniprot.org

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